ArticleThe Journal of cell biology1999
Mcm2, but not RPA, is a component of the mammalian early G1-phase prereplication complex.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
96 citing papers in PubMed, 174 citations in OpenAlex.
- Outside-in progression of heterochromatin replication and exclusion of CDC45 from the PCH domain inbioRxiv : the preprint server for biology · 2026Article
- Transcription elongation can be sufficient, but is not necessary, to advance replication timing.EMBO reports · 2026Article
- Quantity and quality of minichromosome maintenance protein complexes couple replication licensing to genome integrity.Communications biology · 2024Review
- Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.Nature communications · 2022Article
- Article
- FANCD2 limits acetaldehyde-induced genomic instability during DNA replication in esophageal keratinocytes.Molecular oncology · 2021Article
- Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency.Genome biology · 2021Article
- Silencing ofInternational journal of molecular sciences · 2021Article
- Sir2 takes affirmative action to ensure equal opportunity in replication origin licensing.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Inhibition of MCM2 enhances the sensitivity of ovarian cancer cell to carboplatin.Molecular medicine reports · 2019Article
- Coupled cycling programs multicellular self-organization of neural progenitors.Cell cycle (Georgetown, Tex.) · 2019Article
- The replicative helicase MCM recruits cohesin acetyltransferase ESCO2 to mediate centromeric sister chromatid cohesion.The EMBO journal · 2018Article
- Article
- TIMELESS Suppresses the Accumulation of Aberrant CDC45·MCM2-7·GINS Replicative Helicase Complexes on Human Chromatin.The Journal of biological chemistry · 2016Article
- USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication.Molecular oncology · 2016Article
- A unique binding mode enables MCM2 to chaperone histones H3-H4 at replication forks.Nature structural & molecular biology · 2015Article
- Effect of minichromosome maintenance protein 2 deficiency on the locations of DNA replication origins.Genome research · 2015Article
- RAD54 family translocases counter genotoxic effects of RAD51 in human tumor cells.Nucleic acids research · 2015Article
- Dynamic loading and redistribution of the Mcm2-7 helicase complex through the cell cycle.The EMBO journal · 2015Article
- Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication.Cell cycle (Georgetown, Tex.) · 2015Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Previous experiments in Xenopus egg extracts identified what appeared to be two independently assembled prereplication complexes (pre-RCs) for DNA replication: the stepwise assembly of ORC, Cdc6, and Mcm onto chromatin, and the FFA-1-mediated recruitment of RPA into foci on chromatin. We have investigated whether both of these pre-RCs can be detected in Chinese hamster ovary (CHO) cells. Early- and late-replicating chromosomal domains were pulse-labeled with halogenated nucleotides and prelabeled cells were synchronized at various times during the following G1-phase. The recruitment of Mcm2 and RPA to these domains was examined in relation to the formation of a nuclear envelope, specification of the dihydrofolate reductase (DHFR) replication origin and entry into S-phase. Mcm2 was loaded gradually and cumulatively onto both early- and late-replicating chromatin from late telophase throughout G1-phase. During S-phase, detectable Mcm2 was rapidly excluded from PCNA-containing active replication forks. By contrast, detergent-resistant RPA foci were undetectable until the onset of S-phase, when RPA joined only the earliest-firing replicons. During S-phase, RPA was present with PCNA specifically at active replication forks. Together, our data are consistent with a role for Mcm proteins, but not RPA, in the formation of mammalian pre-RCs during early G1-phase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.