ArticleThe Journal of physiology1999
F-actin stabilization increases tension cost during contraction of permeabilized airway smooth muscle in dogs.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Membrane adhesion junctions regulate airway smooth muscle phenotype and function.Physiological reviews · 2023Review
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Pharmacometabolomics of Asthma as a Road Map to Precision Medicine.Handbook of experimental pharmacology · 2023Article
- Effects of TNFα on Dynamic Cytosolic CaFrontiers in physiology · 2021Article
- Cytoskeletal remodeling slows cross-bridge cycling and ATP hydrolysis rates in airway smooth muscle.Physiological reports · 2020Article
- Mechanisms underlying TNFα-induced enhancement of force generation in airway smooth muscle.Physiological reports · 2019Article
- Molecular Mechanisms for the Mechanical Modulation of Airway Responsiveness.Journal of engineering and science in medical diagnostics and therapy · 2019Article
- Endoplasmic Reticulum Stress and Mitochondrial Function in Airway Smooth Muscle.Frontiers in cell and developmental biology · 2019Review
- TNFα decreases mitochondrial movement in human airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- TNFα enhances force generation in airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Biphasic responses of human vascular smooth muscle cells to magnesium ion.Journal of biomedical materials research. Part A · 2016Article
- Interaction between endoplasmic/sarcoplasmic reticulum stress (ER/SR stress), mitochondrial signaling and Ca(2+) regulation in airway smooth muscle (ASM).Canadian journal of physiology and pharmacology · 2015Review
- Prolonged vasoconstriction of resistance arteries involves vascular smooth muscle actin polymerization leading to inward remodelling.Cardiovascular research · 2013Article
- Ca2+ sensitization due to myosin light chain phosphatase inhibition and cytoskeletal reorganization in the myogenic response of skeletal muscle resistance arteries.The Journal of physiology · 2013Article
- The role of actin filament dynamics in the myogenic response of cerebral resistance arteries.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2013Review
- Structure and dynamics of the actin-based smooth muscle contractile and cytoskeletal apparatus.Journal of muscle research and cell motility · 2012Review
- Myosin filament polymerization and depolymerization in a model of partial length adaptation in airway smooth muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Article
- Emergence of airway smooth muscle functions related to structural malleability.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Review
- Pregnancy downregulates actin polymerization and pressure-dependent myogenic tone in ovine uterine arteries.Hypertension (Dallas, Tex. : 1979) · 2010Article
- Epithelial microfilament regulators show regional distribution in mouse conjunctiva.Molecular vision · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
1. Dynamic actin reorganization involving actin polymerization and depolymerization may play an important functional role in smooth muscle. 2. This study tested the hypothesis that F-actin stabilization by phalloidin increases tension cost (i.e. ATP hydrolysis rate per unit of isometric force) during Ca2+-induced activation of Triton X-100-permeabilized canine tracheal smooth muscle. 3. Adenosine 5'-triphosphate (ATP) hydrolysis rate was quantified using an enzyme-coupled NADH fluorometric technique, regulatory myosin light chain (rMLC) phosphorylation was measured by Western blot analysis, and maximum unloaded shortening velocity (Vmax) was estimated by interpolation of the force-velocity relationship to zero load during isotonic loading. 4. Maximal activation with 10 microM free Ca2+ induced sustained increases in isometric force, stiffness, and rMLC phosphorylation. However, the increase in ATP hydrolysis rate initially reached peak values, but then declined to steady-state levels above that of the unstimulated muscle. Thus, tension cost decreased throughout steady-state isometric force. 5. Following incubation of permeabilized strips with 50 microM phalloidin for 1 h, the increases in isometric force and stiffness were not sustained despite a sustained increase in rMLC phosphorylation. Also, after an initial decline, tension cost increased throughout activation. Phalloidin had no effect on Vmax during steady-state isometric force or on rMLC phosphorylation. 6. These findings suggest that dynamic reorganization of actin is necessary for optimal energy utilization during contraction of permeabilized airway smooth muscle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.