ArticleInfection and immunity1999
The EspD protein of enterohemorrhagic Escherichia coli is required for the formation of bacterial surface appendages and is incorporated in the cytoplasmic membranes of target cells.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 83 citations in OpenAlex.
- Class II chaperones: indispensable components of the Gram-negative bacterial T3SS as guardians of translocators.Archives of microbiology · 2026Review
- The sequence of events of enteropathogeniciScience · 2024Article
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- Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels.Bioactive materials · 2021Article
- Transmembrane domains of type III-secreted proteins affect bacterial-host interactions in enteropathogenicVirulence · 2021Article
- Staying out or Going in? The Interplay between Type 3 and Type 5 Secretion Systems in Adhesion and Invasion of Enterobacterial Pathogens.International journal of molecular sciences · 2020Review
- LysR-type transcriptional regulator OvrB encoded in O island 9 drives enterohemorrhagicVirulence · 2019Article
- Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization.PLoS pathogens · 2019Article
- RstA, a two-component response regulator, plays important roles in multiple virulence-associated processes in enterohemorrhagicGut pathogens · 2019Article
- Novel compounds targeting the enterohemorrhagic Escherichia coli type three secretion system reveal insights into mechanisms of secretion inhibition.Molecular microbiology · 2017Article
- The Two-Component System CpxRA Negatively Regulates the Locus of Enterocyte Effacement of Enterohemorrhagic Escherichia coli Involving σ(32) and Lon protease.Frontiers in cellular and infection microbiology · 2016Article
- Type Three Secretion System in Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- Pore-forming Activity of the Escherichia coli Type III Secretion System Protein EspD.The Journal of biological chemistry · 2015Article
- The Serine Protease EspC from Enteropathogenic Escherichia coli Regulates Pore Formation and Cytotoxicity Mediated by the Type III Secretion System.PLoS pathogens · 2015Article
- Virulence gene profiles and population genetic analysis for exploration of pathogenic serogroups of Shiga toxin-producing Escherichia coli.Journal of clinical microbiology · 2013Article
- The ribosome binding site of a mini-ORF protects a T3SS mRNA from degradation by RNase E.Molecular microbiology · 2012Article
- The N-terminal amphipathic region of the Escherichia coli type III secretion system protein EspD is required for membrane insertion and function.Molecular microbiology · 2011Article
- Post-transcriptional processing of the LEE4 operon in enterohaemorrhagic Escherichia coli.Molecular microbiology · 2009Article
- Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array.Journal of clinical microbiology · 2006Article
- Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation.Infection and immunity · 2005Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of EspA-containing surface appendages in pathogenic Escherichia coli strains, both enteropathogenic E. coli (EPEC) and Shiga toxin-producing E. coli strains, is essential for critical events in the infective process, e.g., localized bacterial adherence to host cells with formation of microcolonies and induction of attaching and effacing lesions. It has been reported that EPEC mutants deficient in the production of EspD, which is encoded by the esp operon, are unable to accumulate actin underneath adherent bacteria but exhibit an attachment similar to that of the wild type. Here, we report the construction and characterization of an in-frame espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933. In contrast to what was observed in EPEC mutants, the EDL933 espD mutant not only lacked the capacity to accumulate actin but also exhibited an impaired attachment to HeLa cells. The synthesis of the EspD protein was also essential for the formation of EspA-containing filaments. Finally, localization studies demonstrated that the EspD protein is transferred to the cytoplasm and integrated into the cytoplasmic membranes of infected cells. These results help to elucidate the underlying molecular events in infections caused by EHEC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.