ArticleBritish journal of pharmacology1999
Separation of M-like current and ERG current in NG108-15 cells.
Article in British journal of pharmacology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Evaluation of Small-Molecule Candidates as Modulators of M-Type KInternational journal of molecular sciences · 2025Review
- RLN3/RXFP3 Signaling in the PVN Inhibits Magnocellular Neurons via M-like Current Activation and Contributes to Binge Eating Behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Pharmacological Profiling of Purified Human Stem Cell-Derived and Primary Mouse Motor Neurons.Scientific reports · 2019Article
- Muscarinic Acetylcholine Receptors and M-Currents Underlie Efferent-Mediated Slow Excitation in Calyx-Bearing Vestibular Afferents.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Characterizing the effects of Eugenol on neuronal ionic currents and hyperexcitability.Psychopharmacology · 2012Article
- Evidence for inhibitory effects of flupirtine, a centrally acting analgesic, on delayed rectifier k(+) currents in motor neuron-like cells.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- Ion channels and signaling in the pituitary gland.Endocrine reviews · 2010Review
- HERG1 channelopathies.Pflugers Archiv : European journal of physiology · 2010Review
- Phosphatidylinositol 4,5-bisphosphate interactions with the HERG K(+) channel.Pflugers Archiv : European journal of physiology · 2007Review
- Modulation of hERG potassium currents in HEK-293 cells by protein kinase C. Evidence for direct phosphorylation of pore forming subunits.The Journal of physiology · 2007Article
- M-like K+ currents in type I hair cells and calyx afferent endings of the developing rat utricle.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- KCNQ/M-currents contribute to the resting membrane potential in rat visceral sensory neurons.The Journal of physiology · 2006Article
- Extracellular potassium effects are conserved within the rat erg K+ channel family.The Journal of physiology · 2005Article
- Muscarinic modulation of erg potassium current.The Journal of physiology · 2004Article
- A radiolabeled peptide ligand of the hERG channel, [125I]-BeKm-1.Pflugers Archiv : European journal of physiology · 2003Article
- Functional coupling between heterologously expressed dopamine D(2) receptors and KCNQ channels.Pflugers Archiv : European journal of physiology · 2003Article
- Isolation of a long-lasting eag-related gene-type K+ current in MMQ lactotrophs and its accommodating role during slow firing and prolactin release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Histamine promotes excitability in bovine adrenal chromaffin cells by inhibiting an M-current.The Journal of physiology · 2002Article
- Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiated NG108-15 neuroblastoma x glioma hybrid cells were whole-cell voltage-clamped. Hyperpolarizing pulses, superimposed on a depolarized holding potential (-30 or -20 mV), elicited deactivation currents which consisted of two components, distinguishable by fitting with two exponential functions. Linopirdine [DuP 996, 3,3-bis(4-pyridinylmethyl)-1-phenylindolin-2-one), a neurotransmitter-release enhancer known as potent and selective blocker of the M-current of rat sympathetic neurons, in concentrations of 5 or 10 microM selectively inhibited the fast component (IC50 = 14.7 microM). The slow component was less sensitive to linopirdine (IC50>20 microM). The class III antiarrhythmics [(4-methylsulphonyl)amido]benzenesulphonamide (WAY-123.398) and 1-[2-(6-methyl-2-pyrydinil)ethyl]-4-(4-methylsulphonylaminobenz oyl) piperidine (E-4031), selective inhibitors of the inwardly rectifying ERG (ether-à-go-go-related gene) potassium channel, inhibited predominantly the slow component (IC50 = 38 nM for E-4031). The time constant of the WAY-123.398-sensitive current resembled the time constant of the slow component in size and voltage dependence. Inwardly rectifying ERG currents, recorded in K+ -rich bath at strongly negative pulse potentials, resembled the slow component of the deactivation current in their low sensitivity to linopirdine (28% inhibition at 50 microM). The size of the slow component varied greatly between cells. Accordingly, varied the effect of WAY-123.398 on deactivation current and holding current. RNA transcripts for the following members of the ether-à-go-go gene (EAG) K+ channel family were found in differentiated NG108-15 cells: ERG1, ERG2, EAGI, EAG-like (ELK)1, ELK2; ERG3 was only present in non-differentiated cells. In addition, RNA transcripts for KCNQ2 and KCNQ3 were found in differentiated and non-differentiated cells. We conclude that the fast component of the deactivation current is M-like current and the slow component is deactivating ERG current. The molecular correlates are probably KCNQ2/KCNQ3 and ERG1/ERG2, respectively.
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