Evidence map›Paper›PMID 10455015›Full record

ArticleThe Biochemical journal1999

Activation of TrkA by nerve growth factor upregulates expression of the cholinergic gene locus but attenuates the response to ciliary neurotrophic growth factor.

B Berse, I Lopez-Coviella, J K Blusztajn

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 47 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Peptides Derived from Growth Factors to Treat Alzheimer's Disease.International journal of molecular sciences · 2021
    Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. NGF is essential for hippocampal plasticity and learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009
    Article
  13. Article
  14. Non-cell-autonomous regulation of GABAergic neuron development by neurotrophins and the p75 receptor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007
    Article
  15. Article
  16. Abnormal chemosensory jump 6 is a positive transcriptional regulator of the cholinergic gene locus in Drosophila olfactory neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002
    Article
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

B BerseDepartment of Pathology and Laboratory Medicine, Boston University School of Medicine, 85 East Newton Street, Boston, MA 02118, USA. berse@bu.edu
I Lopez-Coviella
J K Blusztajn
Boston University · US

Funding

TIME FRAMES OF CHOLINE ENHANCEMENT OF MEMORYP01AG009525 · NIA · BOSTON UNIVERSITY MEDICAL CAMPUS · PI BLUSZTAJN, JAN KRZYSZTOF · 1991 to 2010
$11.8M
NIA NIH HHS AG09525
6 · The paper itself

Abstract

Nerve growth factor (NGF) stimulates the expression of the cholinergic gene locus, which encodes choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT), the proteins necessary for the synthesis and storage of the neurotransmitter acetylcholine (ACh). To determine whether this action of NGF is mediated by the p140TrkA NGF receptor (a member of the Trk tyrosine kinase family) we used a murine basal forebrain cholinergic cell line, SN56, stably transfected with rat trkA cDNA. Treatment of these transfectants with NGF activated mitogen-activated protein kinase and increased cytosolic free calcium concentrations, confirming the reconstitution of TrkA-mediated signalling pathways. The expression of ChAT and VAChT mRNA, as well as ACh content, were coordinately up-regulated by NGF in SN56-trkA transfectants. None of these responses occurred in the parental SN56 cells, which do not express endogenous TrkA, indicating that these actions of NGF required TrkA. We previously reported that ciliary neurotrophic factor (CNTF) upregulates the expression of ChAT and VAChT, as well as ACh production, in SN56 cells. The combined treatment of SN56-trkA cells with CNTF and NGF revealed a complex interaction of these factors in the regulation of cholinergic gene locus expression. At low concentrations of CNTF (<1 ng/ml), the upregulation of ACh synthesis evoked by these factors was additive. However, at higher concentrations of CNTF (>1 ng/ml), NGF attenuated the stimulatory effect of CNTF on ChAT and VAChT mRNA and ACh content. This attenuation was not due to interference with early steps of CNTF receptor signalling, as pre-treatment of SN56-trkA cells with NGF did not affect the nuclear translocation of the transcription factor, Stat3, evoked by CNTF.

Indexed as

Carrier ProteinsMembrane Transport ProteinsVesicular Transport ProteinsAcetylcholineAnimalsCalcium SignalingCell LineCholine O-AcetyltransferaseCiliary Neurotrophic FactorDNA-Binding ProteinsMiceNerve Growth FactorRatsReceptors, Nerve Growth FactorRNA, MessengerSignal TransductionAcetylcholineCarrier ProteinsCholine O-AcetyltransferaseCiliary Neurotrophic FactorDNA-Binding ProteinsMembrane Transport ProteinsNerve Growth FactorReceptors, Nerve Growth FactorRNA, MessengerSlc18a3 protein, mouseSlc18a3 protein, ratStat3 protein, mouseStat3 protein, ratSTAT3 Transcription FactorTrans-ActivatorsVesicular Acetylcholine Transport ProteinsVesicular Transport Proteins

Identifiers

PMID10455015
PMCPMC1220465
OpenAlexW2019202087

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.