ArticleNucleic acids research1999
Prevention of depurination during elution facilitates the reamplification of DNA from differential display gels.
Article in Nucleic acids research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells.PloS one · 2020Article
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- Identification of differential expression genes in leaves of rice (Oryza sativa L.) in response to heat stress by cDNA-AFLP analysis.BioMed research international · 2013Article
- Targeted mRNA oxidation regulates sunflower seed dormancy alleviation during dry after-ripening.The Plant cell · 2011Article
- Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates.Mycorrhiza · 2011Article
- The sulfate-rich and extreme saline sediment of the ephemeral tirez lagoon: a biotope for acetoclastic sulfate-reducing bacteria and hydrogenotrophic methanogenic archaea.International journal of microbiology · 2011Article
- Differential profiling of selected defence-related genes induced on challenge with Alternaria brassicicola in resistant white mustard and their comparative expression pattern in susceptible India mustard.Molecular plant pathology · 2008Article
- Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations.Mycorrhiza · 2007Article
- Differential display analysis of gene expression in two immunologically distinct strains of Eimeria maxima.Parasitology research · 2006Article
- Population dynamics within a microbial consortium during growth on diesel fuel in saline environments.Applied and environmental microbiology · 2006Article
- Wood formation from the base to the crown in Pinus radiata: gradients of tracheid wall thickness, wood density, radial growth rate and gene expression.Plant molecular biology · 2006Article
- Dietary zinc modulates gene expression in murine thymus: results from a comprehensive differential display screening.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Differential gene expression in hepatopancreas of the shrimp Metapenaeus ensis during ovarian maturation.Marine biotechnology (New York, N.Y.)Article
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2 authors.
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Abstract
DNA fragments that show a pattern of differential expression on differential display gels must be eluted from the gel matrix and reamplified to enable further analysis. Elution is usually achieved by heating excised gel slices in a small volume of either water or TE. Here we show that this elution step can adversely affect the ability of the eluted DNA to act as a template for PCR reamplification, probably via the process of depurination. Simply switching to an elution solvent designed to minimise depurination (PCR buffer) facilitates the elution of intact DNA fragments. This improvement is likely to be most beneficial when eluting higher molecular weight fragments (e.g. those >500 bp), in situations where the amount of DNA in an excised gel slice is limited or when contaminating differential display products co-migrate with the differentially expressed product.
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