ArticleNucleic acids research1999
The polyoma virus enhancer cannot substitute for DNase I core hypersensitive sites 2-4 in the human beta-globin LCR.
Article in Nucleic acids research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Five nucleotides found in RCTG motifs are essential for post-fertilization methylation imprinting of the H19 ICR in YAC transgenic mice.Nucleic acids research · 2023Article
- The transgenic IG-DMR sequence of the mouse Dlk1-Dio3 domain acquired imprinted DNA methylation during the post-fertilization period.Epigenetics & chromatin · 2023Article
- Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus.Epigenetics & chromatin · 2020Article
- Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice.Epigenetics & chromatin · 2018Article
- Homeostatic Response of MouseMolecular and cellular biology · 2018Article
- Article
- DNase I hypersensitivity and epsilon-globin transcriptional enhancement are separable in locus control region (LCR) HS1 mutant human beta-globin YAC transgenic mice.The Journal of biological chemistry · 2010Article
- All of the human beta-type globin genes compete for LCR enhancer activity in embryonic erythroid cells of yeast artificial chromosome transgenic mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Fine tuning of globin gene expression by DNA methylation.PloS one · 2006Article
- Synergistic and additive properties of the beta-globin locus control region (LCR) revealed by 5'HS3 deletion mutations: implication for LCR chromatin architecture.Molecular and cellular biology · 2005Article
- Adult stage gamma-globin silencing is mediated by a promoter direct repeat element.Molecular and cellular biology · 2005Article
- Human beta-globin locus control region HS5 contains CTCF- and developmental stage-dependent enhancer-blocking activity in erythroid cells.Molecular and cellular biology · 2003Article
- HS5 of the human beta-globin locus control region: a developmental stage-specific border in erythroid cells.The EMBO journal · 2003Article
- Nuclear matrix association of the human beta-globin locus utilizing a novel approach to quantitative real-time PCR.Nucleic acids research · 2003Article
- Polarity of transcriptional enhancement revealed by an insulator element.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Context-dependent EKLF responsiveness defines the developmental specificity of the human epsilon-globin gene in erythroid cells of YAC transgenic mice.Genes & development · 2000Article
- Characteristics of the adeno-associated virus preintegration site in human chromosome 19: open chromatin conformation and transcription-competent environment.Journal of virology · 2000Article
- Deletion of the human beta-globin LCR 5'HS4 or 5'HS1 differentially affects beta-like globin gene expression in beta-YAC transgenic mice.Blood cells, molecules & diseasesArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The polyoma virus enhancer (PyE) is capable of conferring integration position-independent expression to linked genes in stably transfected erythroid cells after joining to DNase I hypersensitive site (HS) 5 of the human beta-globin locus control region (LCR). In attempting to separate the chromatin opening activity of the LCR from its enhancer activity and to investigate contributions of the individual HS core elements to LCR function, the human beta-globin LCR HS2, HS3 and HS4 core elements were replaced with the PyE within the context of a yeast artificial chromosome (YAC) bearing the whole locus. We show here that, in contrast to its function in cultured cells, the PyE is unable to replace HS core element function in vivo. We found that the PyE substitution mutant LCR is unable to provide either chromatin opening or transcriptional potentiating activity at any erythroid developmental stage in transgenic mice. These data provide direct evidence that the human beta-globin LCR core elements specify unique functions that cannot be replaced by a ubiquitous enhancer activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.