ArticleNucleic acids research1999
An origin of bidirectional DNA replication is located within a CpG island at the 3" end of the chicken lysozyme gene.
Article in Nucleic acids research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Epigenetic instability due to defective replication of structured DNA.Molecular cell · 2010Article
- The promoter regions of the Myb-regulated Adora2B and Mcm4 genes co-localize with origins of DNA replication.BMC molecular biology · 2007Article
- Heterochromatin on the inactive X chromosome delays replication timing without affecting origin usage.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The human beta-globin replication initiation region consists of two modular independent replicators.Molecular and cellular biology · 2004Article
- The glioma-amplified sequence 41 gene (GAS41) is a direct Myb target gene.Nucleic acids research · 2004Article
- Replication of the chicken beta-globin locus: early-firing origins at the 5' HS4 insulator and the rho- and betaA-globin genes show opposite epigenetic modifications.Molecular and cellular biology · 2003Article
- The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A Functional chromatin domain does not resist X chromosome inactivation: silencing of cLys correlates with methylation of a dual promoter-replication origin.Molecular and cellular biology · 2002Article
- The chicken lysozyme chromatin domain contains a second, widely expressed gene.Nucleic acids research · 2002Article
- Mammalian Orc1 protein is selectively released from chromatin and ubiquitinated during the S-to-M transition in the cell division cycle.Molecular and cellular biology · 2002Article
- Multiple sites of replication initiation in the human beta-globin gene locus.Nucleic acids research · 2001Article
- Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region.Molecular and cellular biology · 2000Article
- Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals.The EMBO journal · 2000Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously identified a broad initiation zone of DNA replication at the chicken lysozyme gene locus. However, the existence of a highly preferred origin of bidirectional replication (OBR), often found in initiation zones, remained elusive. In order to re-examine this issue we used a competitive PCR assay to determine the abundance of closely spaced genomic segments in a 1 kb size fraction of nascent DNA. A sharp peak of nascent strand abundance occurred at the 3" end of the gene, where initiation events were 17 times more frequent than upstream of the gene. This primary initiation site, active in lysozyme expressing myelomonocytic HD11 cells and non-expressing hepatic DU249 cells, was found to reside within an unusually located CpG island. While most CpG islands are found at the 5" end of genes, the lysozyme gene island extends from the 3" end of the second intron and includes approximately 1.2 kb of 3" flanking DNA. As diagnosed by methylation-sensitive restriction enzymes, the island is largely non-methylated in HD11 cells, DU249 cells and inactive chicken erythrocytes. Furthermore, a DNase I hypersensitive site (HS) that is composed of two subsites separated by approximately 100 bp, was localised very close to the segment with the highest initiation activity. Our results suggest that the non-methylated CpG island and the HS provide an accessible chromatin structure for the lysozyme gene origin of replication.
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