ArticleMolecular and cellular biology1999
Trithorax and ASH1 interact directly and associate with the trithorax group-responsive bxd region of the Ultrabithorax promoter.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- Role of Ash1l in Tourette syndrome and other neurodevelopmental disorders.Developmental neurobiology · 2021Pooled it
- TRITHORAX-dependent arginine methylation of HSP68 mediates circadian repression by PERIOD in the monarch butterfly.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival.Chromosoma · 2021Article
- Mining histone methyltransferases and demethylases from whole genome sequence.Journal of biosciences · 2020Article
- A role of the Trx-G complex in Cid/CENP-A deposition at Drosophila melanogaster centromeres.Chromosoma · 2019Article
- Theoretical analysis of Polycomb-Trithorax systems predicts that poised chromatin is bistable and not bivalent.Nature communications · 2019Article
- Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila.Nature communications · 2017Article
- Review
- The Histone Methyltransferase Gene Absent, Small, or Homeotic Discs-1 Like Is Required for Normal Hox Gene Expression and Fertility in Mice.Biology of reproduction · 2015Article
- Ash1l methylates Lys36 of histone H3 independently of transcriptional elongation to counteract polycomb silencing.PLoS genetics · 2013Article
- Stepwise histone modifications are mediated by multiple enzymes that rapidly associate with nascent DNA during replication.Nature communications · 2013Article
- The Arabidopsis thaliana SET-domain-containing protein ASHH1/SDG26 interacts with itself and with distinct histone lysine methyltransferases.Journal of plant research · 2012Article
- Crystal structure of the human histone methyltransferase ASH1L catalytic domain and its implications for the regulatory mechanism.The Journal of biological chemistry · 2011Article
- Histone H3 lysine 4 (H3K4) methylation in development and differentiation.Developmental biology · 2010Review
- Alternative epigenetic chromatin states of polycomb target genes.PLoS genetics · 2010Article
- The ASH1 HOMOLOG 2 (ASHH2) histone H3 methyltransferase is required for ovule and anther development in Arabidopsis.PloS one · 2009Article
- Histone demethylases and cancer.Advances in cancer research · 2009Review
- dKDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing.Genes & development · 2008Article
- The trithorax group and Pc group proteins are differentially involved in heterochromatin formation in Drosophila.Chromosoma · 2008Article
- Mammalian ASH1L is a histone methyltransferase that occupies the transcribed region of active genes.Molecular and cellular biology · 2007Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
Trithorax (TRX) and ASH1 belong to the trithorax group (trxG) of transcriptional activator proteins, which maintains homeotic gene expression during Drosophila development. TRX and ASH1 are localized on chromosomes and share several homologous domains with other chromatin-associated proteins, including a highly conserved SET domain and PHD fingers. Based on genetic interactions between trx and ash1 and our previous observation that association of the TRX protein with polytene chromosomes is ash1 dependent, we investigated the possibility of a physical linkage between the two proteins. We found that the endogenous TRX and ASH1 proteins coimmunoprecipitate from embryonic extracts and colocalize on salivary gland polytene chromosomes. Furthermore, we demonstrated that TRX and ASH1 bind in vivo to a relatively small (4 kb) bxd subregion of the homeotic gene Ultrabithorax (Ubx), which contains several trx response elements. Analysis of the effects of ash1 mutations on the activity of this regulatory region indicates that it also contains ash1 response element(s). This suggests that ASH1 and TRX act on Ubx in relatively close proximity to each other. Finally, TRX and ASH1 appear to interact directly through their conserved SET domains, based on binding assays in vitro and in yeast and on coimmunoprecipitation assays with embryo extracts. Collectively, these results suggest that TRX and ASH1 are components that interact either within trxG protein complexes or between complexes that act in close proximity on regulatory DNA to maintain Ubx transcription.
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