ArticleMolecular and cellular biology1999
Phosphorylation-independent inhibition of Cdc28p by the tyrosine kinase Swe1p in the morphogenesis checkpoint.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 67 citations in OpenAlex.
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- Kinetic partitioning during de novo septin filament assembly creates a critical G1 "window of opportunity" for mutant septin function.Cell cycle (Georgetown, Tex.) · 2016Article
- Sensing a bud in the yeast morphogenesis checkpoint: a role for Elm1.Molecular biology of the cell · 2016Article
- Budding yeast Swe1 is involved in the control of mitotic spindle elongation and is regulated by Cdc14 phosphatase during mitosis.The Journal of biological chemistry · 2015Article
- Comparative genetic analysis of PP2A-Cdc55 regulators in budding yeast.Cell cycle (Georgetown, Tex.) · 2014Article
- The transmission of nuclear pore complexes to daughter cells requires a cytoplasmic pool of Nsp1.The Journal of cell biology · 2013Article
- Cell cycle checkpoint regulators reach a zillion.Cell cycle (Georgetown, Tex.) · 2013Review
- Feedback control of Swe1p degradation in the yeast morphogenesis checkpoint.Molecular biology of the cell · 2013Article
- Roles of Hsl1p and Hsl7p in Swe1p degradation: beyond septin tethering.Eukaryotic cell · 2012Article
- Zds1 regulates PP2A(Cdc55) activity and Cdc14 activation during mitotic exit through its Zds_C motif.Journal of cell science · 2012Article
- Article
- The budding yeast polo-like kinase Cdc5 regulates the Ndt80 branch of the meiotic recombination checkpoint pathway.Molecular biology of the cell · 2011Article
- Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast.The Journal of cell biology · 2011Article
- The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase.Molecular biology of the cell · 2011Article
- Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain.Molecular biology of the cell · 2010Article
- Hsp90 phosphorylation, Wee1 and the cell cycle.Cell cycle (Georgetown, Tex.) · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
The morphogenesis checkpoint in budding yeast delays cell cycle progression in G(2) when the actin cytoskeleton is perturbed, providing time for cells to complete bud formation prior to mitosis. Checkpoint-induced G(2) arrest involves the inhibition of the master cell cycle regulatory cyclin-dependent kinase, Cdc28p, by the Wee1 family kinase Swe1p. Results of experiments using a nonphosphorylatable CDC28(Y19F) allele suggested that the checkpoint stimulated two inhibitory pathways, one that promoted phosphorylation at tyrosine 19 (Y19) and a poorly characterized second pathway that did not require Cdc28p Y19 phosphorylation. We present the results from a genetic screen for checkpoint-defective mutants that led to the repeated isolation of the dominant CDC28(E12K) allele that is resistant to Swe1p-mediated inhibition. Comparison of this allele with the nonphosphorylatable CDC28(Y19F) allele suggested that Swe1p is still able to inhibit CDC28(Y19F) in a phosphorylation-independent manner and that both the Y19 phosphorylation-dependent and -independent checkpoint pathways in fact reflect Swe1p inhibition of Cdc28p. Remarkably, we found that a Swe1p mutant lacking catalytic activity could significantly delay the cell cycle in vivo during a physiological checkpoint response, even when expressed at single copy. The finding that a Wee1 family kinase expressed at physiological levels can inhibit a nonphosphorylatable cyclin-dependent kinase has broad implications for many checkpoint studies using such mutants in other organisms.
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