ArticleMolecular and cellular biology1999
Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae reveal a specific role in meiosis.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- Mammalian tRNA acetylation determines translation efficiency and tRNA quality control.Nature communications · 2025Article
- Characterization of exoribonuclease XRN1 as a cancer target and identification of adenosine-3',5'-bisphosphate as a potent enzyme inhibitor.Communications biology · 2025Article
- Article
- Evidence that Xrn1 is in complex with Gcn1, and is required for full levels of eIF2α phosphorylation.The Biochemical journal · 2024Article
- Retroelement decay by the exonuclease XRN1 is a viral mimicry dependency in cancer.Cell reports · 2024Article
- Article
- Association of ScV-LA Virus with Host Protein Metabolism Determined by Proteomics Analysis and Cognate RNA Sequencing.Viruses · 2022Article
- Observation of conformational changes that underlie the catalytic cycle of Xrn2.Nature chemical biology · 2022Article
- Adaptive Response ofJournal of fungi (Basel, Switzerland) · 2022Article
- Endogenous RNAi pathway evolutionarily shapes the destiny of the antisense lncRNAs transcriptome.Life science alliance · 2019Article
- Small changes, big implications: The impact of mBiochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
- The exonuclease Xrn1 activates transcription and translation of mRNAs encoding membrane proteins.Nature communications · 2019Article
- Article
- Distinct and redundant roles of exonucleases in Cryptococcus neoformans: implications for virulence and mating.Fungal genetics and biology : FG & B · 2014Article
- Engineering of a conditional allele reveals multiple roles of XRN2 in Caenorhabditis elegans development and substrate specificity in microRNA turnover.Nucleic acids research · 2014Article
- Cytoplasmic 5'-3' exonuclease Xrn1p is also a genome-wide transcription factor in yeast.Frontiers in genetics · 2014Article
- Ribosome biogenesis requires a highly diverged XRN family 5'->3' exoribonuclease for rRNA processing in Trypanosoma brucei.RNA (New York, N.Y.) · 2013Article
- Activation of 5'-3' exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Unpairing and gating: sequence-independent substrate recognition by FEN superfamily nucleases.Trends in biochemical sciences · 2012Review
- The wonders of flap endonucleases: structure, function, mechanism and regulation.Sub-cellular biochemistry · 2012Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xrn1p of Saccharomyces cerevisiae is a major cytoplasmic RNA turnover exonuclease which is evolutionarily conserved from yeasts to mammals. Deletion of the XRN1 gene causes pleiotropic phenotypes, which have been interpreted as indirect consequences of the RNA turnover defect. By sequence comparisons, we have identified three loosely defined, common 5'-3' exonuclease motifs. The significance of motif II has been confirmed by mutant analysis with Xrn1p. The amino acid changes D206A and D208A abolish singly or in combination the exonuclease activity in vivo. These mutations show separation of function. They cause identical phenotypes to that of xrn1Delta in vegetative cells but do not exhibit the severe meiotic arrest and the spore lethality phenotype typical for the deletion. In addition, xrn1-D208A does not cause the severe reduction in meiotic popout recombination in a double mutant with dmc1 as does xrn1Delta. Biochemical analysis of the DNA binding, exonuclease, and homologous pairing activity of purified mutant enzyme demonstrated the specific loss of exonuclease activity. However, the mutant enzyme is competent to promote in vitro assembly of tubulin into microtubules. These results define a separable and specific function of Xrn1p in meiosis which appears unrelated to its RNA turnover function in vegetative cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.