ArticlePharmaceutical research1999
Intracellular compartmentalization of DNA fragments in cultured airway epithelial cells mediated by cationic lipids.
Article in Pharmaceutical research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Copy number of naked DNA delivered into nucleus of mammalian cells by electrotransfection.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Rheological Properties of DNA Molecules in Solution: Molecular Weight and Entanglement Influences.Polymers · 2016Article
- Investigation of Polyethylenimine/DNA Polyplex Transfection to Cultured Cells Using Radiolabeling and Subcellular Fractionation Methods.Molecular pharmaceutics · 2013Article
- Barriers to non-viral vector-mediated gene delivery in the nervous system.Pharmaceutical research · 2011Review
- Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Intracellular trafficking of nonviral vectors.Gene therapy · 2005Review
- DNA-based therapeutics and DNA delivery systems: a comprehensive review.The AAPS journal · 2005Review
- Sequence-specific modification of genomic DNA by small DNA fragments.The Journal of clinical investigation · 2003Review
- Extrachromosomal genes: a powerful tool in gene targeting approaches.Gene therapy · 2002Article
- Application of SFHR to gene therapy of monogenic disorders.Gene therapy · 2002Article
- Quantitative analysis of correlation between number of nuclear plasmids and gene expression activity after transfection with cationic liposomes.Pharmaceutical research · 2002Article
- Expression of DeltaF508 CFTR in normal mouse lung after site-specific modification of CFTR sequences by SFHR.Gene therapy · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
purposeThe amount and intracellular distribution of DNA fragments (491-bp) was characterized after transfection in vitro with a commercially available cationic lipid. Localization of fragment to the nucleus, its subcellular distribution, and integrity within the cells was determined for various times after transfection.
methodsCystic fibrosis (CF) airway epithelial cells were transfected with 32P and FITC labeled single-stranded (ss) or double-stranded (ds) DNA fragments complexed with Lipofectamine at various charge ratios.
resultsA 511 (+/-) charge ratio was found to be the optimal ratio for transfection of both ss-and dsDNA. After a 5 h exposure, 7.51 +/- 0.89% of the radioactivity was associated with the nuclear fraction whereas only 1.07 +/- 0.23%, was found in the nuclear fraction when dsDNA was used. The nuclear radioactivity detected after a 24 h exposure was only 1/3 of that after 5 h. Analysis of fragment stability in the cytosolic and nuclear fractions showed the presence of intact fragment in each subcellular compartment. No intranuclear/intracellular fragment could be detected in control experiments with naked DNA. Conclusions. The results from these experiments indicate that small fragments of DNA can be efficiently and rapidly transferred intact to the cell nucleus using cationic lipids and that ssDNA fragments are more effective than dsDNA fragments for nuclear delivery.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.