ArticleAnnals of surgery1999
Reduction of ischemia-reperfusion injury of the liver by in vivo adenovirus-mediated gene transfer of the antiapoptotic Bcl-2 gene.
Article in Annals of surgery, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 141 citations in OpenAlex.
- Exploring cell death mechanisms in liver transplantation: implications for graft survival.Frontiers in immunology · 2025Review
- Redox-sensitive epigenetic activation of SUV39H1 contributes to liver ischemia-reperfusion injury.Redox biology · 2024Article
- An Evaluation of the Effectiveness of Melatonin and n-Acetylcysteine in Cerebral Ischemia-Reperfusion Injury in Adult Rats.Medicina (Kaunas, Lithuania) · 2023Article
- Drug delivery nanosystems targeted to hepatic ischemia and reperfusion injury.Drug delivery and translational research · 2021Review
- DNAX Activating Protein of 12 kDa/Triggering Receptor Expressed on Myeloid Cells 2 Expression by Mouse and Human Liver Dendritic Cells: Functional Implications and Regulation of Liver Ischemia-Reperfusion Injury.Hepatology (Baltimore, Md.) · 2019Article
- Recombinant relaxin protects liver transplants from ischemia damage by hepatocyte glucocorticoid receptor: From bench-to-bedside.Hepatology (Baltimore, Md.) · 2018Article
- Reviews of Interleukin-37: Functions, Receptors, and Roles in Diseases.BioMed research international · 2018Review
- Salidroside pretreatment attenuates apoptosis and autophagy during hepatic ischemia-reperfusion injury by inhibiting the mitogen-activated protein kinase pathway in mice.Drug design, development and therapy · 2017Article
- Quercetin Pretreatment Attenuates Hepatic Ischemia Reperfusion-Induced Apoptosis and Autophagy by Inhibiting ERK/NF-Gastroenterology research and practice · 2017Article
- Bcl2 is a critical regulator of bile acid homeostasis by dictating Shp and lncRNA H19 function.Scientific reports · 2016Article
- Upregulation of p21 activates the intrinsic apoptotic pathway in β-cells.American journal of physiology. Endocrinology and metabolism · 2013Article
- Apoptosis and necrosis in the liver.Comprehensive Physiology · 2013Review
- Tissue damage and oxidant/antioxidant balance.The Eurasian journal of medicine · 2013Review
- Immediately transcripted genes in various hepatic ischemia models.Journal of the Korean Surgical Society · 2012Article
- Interleukin-33 is hepatoprotective during liver ischemia/reperfusion in mice.Hepatology (Baltimore, Md.) · 2012Article
- Hypoxic hepatitis: a challenging diagnosis.Hepatology international · 2012Article
- Interleukin-37 reduces liver inflammatory injury via effects on hepatocytes and non-parenchymal cells.Journal of gastroenterology and hepatology · 2012Article
- Receptor activator of nuclear factor-κB ligand (RANKL) protects against hepatic ischemia/reperfusion injury in mice.Hepatology (Baltimore, Md.) · 2012Article
- Anti-apoptotic and antioxidant effect of leptin on CCl₄-induced acute liver injury in rats.Molecular biology reports · 2012Article
- Canine liver transplantation model and the intermediate filaments of the cytoskeleton of the hepatocytes.Journal of biomedicine & biotechnology · 2012Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
objectiveTo examine the possibility of reducing ischemia-reperfusion injury (I/R injury) to the mouse liver by in vivo adenovirus-mediated gene transfer of the antiapoptotic human Bcl-2 gene. SUMMARY BACKGROUND DATA: Ischemia-reperfusion injury has been demonstrated in a number of clinically relevant diseases such as myocardial infarction, cerebrovascular disease, sepsis, peripheral vascular disease, and organ transplantation. In this regard, apoptosis plays a central role.
methodsNormal C57BL/6 mice were used. An adenovirus (deltaE1) vector containing the human Bcl-2 gene was developed in the authors' laboratory. An adenovirus vector encoding an irrelevant gene (beta-galactosidase, AdCMVLacZ) was used as a control. Taking advantage of the hepatotropic properties of adenovirus vectors, gene transfer was performed with 1 x 10(9) plaque-forming units by intravenous tail injection, 48 hours before the ischemic injury. Ischemic-reperfusion injury was induced by temporal and segmental occlusion of hepatic blood flow. Aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase activity was measured using standard assays. Liver biopsies were obtained before and 6 hours after I/R injury for morphologic assessment, and apoptosis was determined in situ with a histochemical assay.
resultsThe expression of AdCMVhBcl-2 vector was confirmed by reverse transcription-polymerase chain reaction and functionally validated in apoptotic studies in endothelial cells. Expression of the Bcl-2 gene protects against I/R injury, as shown by a significant decrease in transaminases (p < 0.05) and necrosis and apoptosis (p < 0.001), and permanent survival (p < 0.0001), compared with sham-operated animals and animals treated with AdCMVLacZ.
conclusionsGenetic modification of the liver to induce cytoprotection has potential applications to prevent I/R injury to the liver in surgical interventions, including liver transplantation.
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