ArticleProceedings of the National Academy of Sciences of the United States of America1999
Protochlorophyllide oxidoreductase B-catalyzed protochlorophyllide photoreduction in vitro: insight into the mechanism of chlorophyll formation in light-adapted plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- Arabidopsis GOLDEN2-LIKE transcription factors interact with ABA-INSENSITIVE 4 to modulate cotyledon greening in light-exposed etiolated seedlings.Journal of experimental botany · 2026Article
- Light dependent protochlorophyllide oxidoreductase: a succinct look.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Enzymes degraded under high light maintain proteostasis by transcriptional regulation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- NADPH:protochlorophyllide oxidoreductase B (PORB) action in Arabidopsis thaliana revisited through transgenic expression of engineered barley PORB mutant proteins.Plant molecular biology · 2017Article
- Wavelength-dependent photooxidation and photoreduction of protochlorophyllide and protochlorophyll in the innermost leaves of cabbage (Brassica oleracea var. capitata L.).Photosynthesis research · 2016Article
- Cell growth defect factor 1 is crucial for the plastid import of NADPH:protochlorophyllide oxidoreductase A in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Dark-operative protochlorophyllide oxidoreductase generates substrate radicals by an iron-sulphur cluster in bacteriochlorophyll biosynthesis.Scientific reports · 2014Article
- Dominance of a 675 nm chlorophyll(ide) form upon selective 632.8 or 654 nm laser illumination after partial protochlorophyllide phototransformation.Photosynthesis research · 2012Article
- Early and late plastid development in response to chill stress and heat stress in wheat seedlings.Protoplasma · 2011Article
- Article
- Photoprotective role of NADPH:protochlorophyllide oxidoreductase A.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Differential operation of dual protochlorophyllide reductases for chlorophyll biosynthesis in response to environmental oxygen levels in the cyanobacterium Leptolyngbya boryana.Plant physiology · 2006Article
- In vitro-mutagenesis of NADPH:protochlorophyllide oxidoreductase B: two distinctive protochlorophyllide binding sites participate in enzyme catalysis and assembly.Molecular genetics and genomics : MGG · 2006Article
- Spectroscopic and kinetic characterization of the light-dependent enzyme protochlorophyllide oxidoreductase (POR) using monovinyl and divinyl substrates.The Biochemical journal · 2006Article
- Novel Insights into the Enzymology, Regulation and Physiological Functions of Light-dependent Protochlorophyllide Oxidoreductase in Angiosperms.Photosynthesis research · 2004Article
- Enzymology below 200 K: the kinetics and thermodynamics of the photochemistry catalyzed by protochlorophyllide oxidoreductase.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- POR structural domains important for the enzyme activity in R. capsulatus complementation system.Photosynthesis research · 2002Article
- The charge-transfer complex between protochlorophyllide and NADPH: an intermediate in protochlorophyllide photoreduction.Photosynthesis research · 2002Article
- A new pathway of chlorophyll biosynthesis from long-wavelength protochlorophyllide Pchlide 686/676 in juvenile etiolated plants.Photosynthesis research · 2002Article
- The protochlorophyllide-chlorophyllide cycle.Photosynthesis research · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The mechanism of the protochlorophyllide (PChlide) photoreduction reaction operating in light-adapted plants and catalyzed by NADPH:protochlorophyllide oxidoreductase B (PORb) has been analyzed by low-temperature fluorescence spectroscopy by using purified barley PORb overexpressed heterologously in Escherichia coli as a fusion protein with the maltose-binding protein. We show that the PORb-catalyzed PChlide reduction reaction consists of two steps, one photochemical and the other nonphotochemical. The initial photochemical reaction follows a single quantum mechanism and leads to the formation of an unstable intermediate with mixed pigment electronic structure and an EPR spectrum that suggests the presence of a free electron. The second step involves the spontaneous conversion of the unstable intermediate into chlorophyllide as defined by its spectroscopic characteristics and migration on an HPLC column. Both steps of the reaction can be performed at subzero temperatures in frozen samples, suggesting that they do not include major changes in enzyme conformation or pigment rearrangement within the active site. The rate of the reaction at room temperature depends linearly on enzyme and substrate (PChlide) concentration, and the kinetic parameters are consistent with one molecule of substrate bound per active monomer in solution. The PORb-catalyzed reaction in vitro is spectroscopically similar to that identified in leaves of light-adapted plants, suggesting that the same reaction sequence observed operates in planta.
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