ArticleProceedings of the National Academy of Sciences of the United States of America1999
Functional characterization of B lymphocytes generated in vitro from embryonic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- In vitro differentiation of common lymphoid progenitor cells into B cell using stromal cell free culture system.BMC immunology · 2025Article
- Terminal deoxynucleotidyl transferase and CD84 identify human multi-potent lymphoid progenitors.Nature communications · 2024Article
- Microbial ligand-independent regulation of lymphopoiesis by NOD1.Nature immunology · 2023Article
- Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical application.Stem cell research & therapy · 2023Review
- Realization of the T Lineage Program Involves GATA-3 Induction of Bcl11b and Repression of Cdkn2b Expression.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- CRLF2 overexpression results in reduced B-cell differentiation and upregulated E2F signaling in the Dp16 mouse model of Down syndrome.Experimental hematology · 2022Article
- Regeneration of immunocompetent B lymphopoiesis from pluripotent stem cells guided by transcription factors.Cellular & molecular immunology · 2022Article
- Vertically transferred maternal immune cells promote neonatal immunity against early life infections.Nature communications · 2021Article
- Trajectory of chemical cocktail-induced neutrophil reprogramming.Journal of hematology & oncology · 2020Article
- Beyond the CRAC: Diversification of ion signaling in B cells.Immunological reviews · 2019Review
- Development of innate immune cells from human pluripotent stem cells.Experimental hematology · 2019Review
- Review
- Control of HIV Infection In Vivo Using Gene Therapy with a Secreted Entry Inhibitor.Molecular therapy. Nucleic acids · 2017Article
- Generation of an osteoblast-based artificial niche that supports in vitro B lymphopoiesis.Experimental & molecular medicine · 2017Article
- PLCγ-dependent mTOR signalling controls IL-7-mediated early B cell development.Nature communications · 2017Article
- Effects of autologous stromal cells and cytokines on differentiation of equine bone marrow-derived progenitor cells.American journal of veterinary research · 2017Article
- Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.Stem cell reports · 2017Article
- Lymphoid differentiation of hematopoietic stem cells requires efficient Cxcr4 desensitization.The Journal of experimental medicine · 2017Article
- The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis.PLoS genetics · 2017Article
- MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study molecular events involved in B lymphocyte development and V(D)J rearrangement, we have established an efficient system for the differentiation of embryonic stem (ES) cells into mature Ig-secreting B lymphocytes. Here, we show that B lineage cells generated in vitro from ES cells are functionally analogous to normal fetal liver-derived or bone marrow-derived B lineage cells at three important developmental stages: first, they respond to Flt-3 ligand during an early lymphopoietic progenitor stage; second, they become targets for Abelson murine leukemia virus (A-MuLV) infection at a pre-B cell stage; third, they secrete Ig upon stimulation with lipopolysaccharide at a mature mitogen-responsive stage. Moreover, the ES cell-derived A-MuLV-transformed pre-B (EAB) cells are phenotypically and functionally indistinguishable from standard A-MuLV-transformed pre-B cells derived from infection of mouse fetal liver or bone marrow. Notably, EAB cells possess functional V(D)J recombinase activity. In particular, the generation of A-MuLV transformants from ES cells will provide an advantageous system to investigate genetic modifications that will help to elucidate molecular mechanisms in V(D)J recombination and in A-MuLV-mediated transformation.
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