ArticleProceedings of the National Academy of Sciences of the United States of America1999
Sequence-tagged connectors: a sequence approach to mapping and scanning the human genome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Generation of physical map contig-specific sequences.Frontiers in genetics · 2014Review
- Advances in BAC-based physical mapping and map integration strategies in plants.Journal of biomedicine & biotechnology · 2012Review
- Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome.Journal of experimental botany · 2011Article
- LTC: a novel algorithm to improve the efficiency of contig assembly for physical mapping in complex genomes.BMC bioinformatics · 2010Article
- An overview of the apple genome through BAC end sequence analysis.Plant molecular biology · 2008Article
- Progress in understanding and sequencing the genome of Brassica rapa.International journal of plant genomics · 2008Article
- End-sequencing and characterization of silkworm (Bombyx mori) bacterial artificial chromosome libraries.BMC genomics · 2007Article
- Construction of a nurse shark (Ginglymostoma cirratum) bacterial artificial chromosome (BAC) library and a preliminary genome survey.BMC genomics · 2006Article
- Pooled genomic indexing of rhesus macaque.Genome research · 2005Article
- Human, mouse, and rat genome large-scale rearrangements: stability versus speciation.Genome research · 2004Article
- An integrated 4249 marker FISH/RH map of the canine genome.BMC genomics · 2004Article
- Construction of a BAC library of Korean ginseng and initial analysis of BAC-end sequences.Molecular genetics and genomics : MGG · 2004Article
- End-sequence profiling: sequence-based analysis of aberrant genomes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A 1-Mb resolution radiation hybrid map of the canine genome.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Mapping by sequencing the Pneumocystis genome using the ordering DNA sequences V3 tool.Genetics · 2003Article
- Construction of a BAC library and generation of BAC end sequence-tagged connectors for genome sequencing of the African malaria mosquito Anopheles gambiae.Molecular genetics and genomics : MGG · 2003Article
- A genetic analysis of chromosome territory looping: diverse roles for distal regulatory elements.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- Article
- A bacterial artificial chromosome library for sequencing the complete human genome.Genome research · 2001Article
- Initial sequencing and analysis of the human genome.Nature · 2001Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sequence-tagged connector (STC) strategy proposes to generate sequence tags densely scattered (every 3.3 kilobases) across the human genome by arraying 450,000 bacterial artificial chromosomes (BACs) with randomly cleaved inserts, sequencing both ends of each, and preparing a restriction enzyme fingerprint of each. The STC resource, containing end sequences, fingerprints, and arrayed BACs, creates a map where the interrelationships of the individual BAC clones are resolved through their STCs as overlapping BAC clones are sequenced. Once a seed or initiation BAC clone is sequenced, the minimum overlapping 5' and 3' BAC clones can be identified computationally and sequenced. By reiterating this "sequence-then-map by computer analysis against the STC database" strategy, a minimum tiling path of clones can be sequenced at a rate that is primarily limited by the sequencing throughput of individual genome centers. As of February 1999, we had deposited, together with The Institute for Genomic Research (TIGR), into GenBank 314,000 STCs ( approximately 135 megabases), or 4.5% of human genomic DNA. This genome survey reveals numerous genes, genome-wide repeats, simple sequence repeats (potential genetic markers), and CpG islands (potential gene initiation sites). It also illustrates the power of the STC strategy for creating minimum tiling paths of BAC clones for large-scale genomic sequencing. Because the STC resource permits the easy integration of genetic, physical, gene, and sequence maps for chromosomes, it will be a powerful tool for the initial analysis of the human genome and other complex genomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.