ArticleProceedings of the National Academy of Sciences of the United States of America1999
Human eosinophils regulate human lung- and skin-derived fibroblast properties in vitro: a role for transforming growth factor beta (TGF-beta).
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 204 citations in OpenAlex.
- Anti-IL-5 treatment reduces deposition of ECM proteins in the bronchial subepithelial basement membrane of mild atopic asthmatics.The Journal of clinical investigation · 2003Trial
- Fibrotic chronic eosinophilic pneumonia: from inflammation to fibrosis and therapeutic implications.European respiratory review : an official journal of the European Respiratory Society · 2026Review
- Eosinophil-associated matrix remodeling in a sterile granulomatous inflammation model: a temporal histopathological analysis.Histochemistry and cell biology · 2026Article
- Age-related increase of NLRC4 inflammasome and TGF-β1 signaling exacerbates airway inflammation and remodeling in asthma.GeroScience · 2026Article
- Eosinophils as immunoregulatory players in allergic rhinitis: beyond cytotoxicity.Frontiers in immunology · 2026Review
- Emerging Roles of Eosinophils in Bone.Current osteoporosis reports · 2025Review
- Chronic Lung Allograft Dysfunction: Clinical Manifestations and Immunologic Mechanisms.Transplantation · 2025Review
- Clinical, mechanistic, and therapeutic landscape of cutaneous fibrosis.Science translational medicine · 2024Review
- The action of topical application of Vitamin BInternational wound journal · 2023Article
- Could the Epigenetics of Eosinophils in Asthma and Allergy Solve Parts of the Puzzle?International journal of molecular sciences · 2021Review
- COMP: A Potential Early Biomarker of RAS After Lung Transplantation.Transplantation direct · 2021Article
- A case of juvenile eosinophilic cholangitis: Rapid peripheral blood hypereosinophilia after admission leading to diagnosis.JGH open : an open access journal of gastroenterology and hepatology · 2021Article
- Eosinophils in Eosinophilic Esophagitis: The Road to Fibrostenosis is Paved With Good Intentions.Frontiers in immunology · 2020Review
- Basophils and Eosinophils in Nematode Infections.Frontiers in immunology · 2020Review
- The Role of Autophagy in Eosinophilic Airway Inflammation.Immune network · 2019Review
- Structure Identification of ViceninII Extracted fromMolecules (Basel, Switzerland) · 2019Article
- Review
- Obesity-induced reduction of adipose eosinophils is reversed with low-calorie dietary intervention.Physiological reports · 2018Article
- Activation of Fibroblast Contractility via Cell-Cell Interactions and Soluble Signals.Bio-protocol · 2018Article
- Shaping eosinophil identity in the tissue contexts of development, homeostasis, and disease.Journal of leukocyte biology · 2018Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eosinophils have been associated with fibrosis. To investigate their direct role in fibrosis, human peripheral blood eosinophil sonicate was added to human lung or dermal fibroblasts, and proliferation ([(3)H]thymidine) and collagen synthesis ([(3)H]proline) were evaluated. Proliferation was enhanced significantly in the monolayers in a dose-dependent manner. The activity of the eosinophil fibrogenic factor(s) remained unaltered when heated (56 degrees C, 30 min). Supernatants of cultured eosinophils (20 min or 18 hr) also enhanced lung fibroblast proliferation, indicating that the preformed mitogenic factor(s) can be released both promptly and with a long kinetic. Eosinophils significantly decreased collagen production in lung fibroblasts while increasing it in dermal fibroblasts. However, eosinophils containing matrix metalloproteinase 9 (zymography) in latent form and tissue inhibitors of metalloproteinases 1 and 2 (reverse zymography) did not influence either fibroblast matrix metalloproteinases or tissue inhibitors of metalloproteinases. Eosinophil sonicate added to skin and lung fibroblasts in tridimensional collagen lattices significantly enhanced lattice contraction. Transforming growth factor beta (TGF-beta) is a major fibrogenic cytokine produced by eosinophils. Therefore, to assess its role, eosinophil sonicate was preincubated with anti-TGF-beta neutralizing antibodies. This treatment partially inhibited proliferation of lung and collagen synthesis of dermal fibroblasts and suppressed the stimulation of lattice contraction, indicating the fibrogenic role of eosinophil-associated TGF-beta. In conclusion, we have shown that eosinophils act as direct modulatory cells in fibroblast proliferation, collagen synthesis, and lattice contraction, in part, through TGF-beta. These data corroborate the importance of eosinophils in skin and lung fibrosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.