ArticleProceedings of the National Academy of Sciences of the United States of America1999
Imaging of thermal activation of actomyosin motors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- A stochastic mechanism drives fast substrate translocation in the AAA+ machine ClpB.Nature communications · 2026Article
- Actomyosin-Based Nanodevices for Sensing and Actuation: Bridging Biology and Bioengineering.Biosensors · 2025Review
- Myosin and tropomyosin-troponin complementarily regulate thermal activation of muscles.The Journal of general physiology · 2023Article
- Trans-scale thermal signaling in biological systems.Journal of biochemistry · 2023Review
- Modulation of Local Cellular Activities using a Photothermal Dye-Based Subcellular-Sized Heat Spot.ACS nano · 2022Article
- Opto-thermal technologies for microscopic analysis of cellular temperature-sensing systems.Biophysical reviews · 2022Review
- GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.The Journal of cell biology · 2021Article
- Temperature-Dependent Activity of Motor Proteins: Energetics and Their Implications for Collective Behavior.Frontiers in cell and developmental biology · 2021Review
- Absorption cross section of gold nanoparticles based on NIR laser heating and thermodynamic calculations.Scientific reports · 2020Article
- Microscopic heat pulses activate cardiac thin filaments.The Journal of general physiology · 2019Article
- Local heating of molecular motors using single carbon nanotubes.Biophysical reviews · 2016Review
- Triggering of high-speed neurite outgrowth using an optical microheater.Scientific reports · 2015Article
- Directional bleb formation in spherical cells under temperature gradient.Biophysical journal · 2015Article
- Probing DNA helicase kinetics with temperature-controlled magnetic tweezers.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Impact of heating on passive and active biomechanics of suspended cells.Interface focus · 2014Article
- Micromechanical thermal assays of Ca2+-regulated thin-filament function and modulation by hypertrophic cardiomyopathy mutants of human cardiac troponin.Journal of biomedicine & biotechnology · 2012Article
- Two methods of temperature control for single-molecule measurements.European biophysics journal : EBJ · 2011Article
- Simultaneous pH and temperature measurements using pyranine as a molecular probe.Journal of fluorescence · 2011Article
- Real-Time Infrared Overtone Laser Control of Temperature in Picoliter H(2)O Samples: "Nanobathtubs" for Single Molecule Microscopy.The journal of physical chemistry letters · 2010Article
- Watching individual proteins acting on single molecules of DNA.Methods in enzymology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed temperature-pulse microscopy in which the temperature of a microscopic sample is raised reversibly in a square-wave fashion with rise and fall times of several ms, and locally in a region of approximately 10 micrometers in diameter with a temperature gradient up to 2 degrees C/micrometers. Temperature distribution was imaged pixel by pixel by image processing of the fluorescence intensity of rhodamine phalloidin attached to (single) actin filaments. With short pulses, actomyosin motors could be activated above physiological temperatures (higher than 60 degrees C at the peak) before thermally induced protein damage began to occur. When a sliding actin filament was heated to 40-45 degrees C, the sliding velocity reached 30 micrometers/s at 25 mM KCl and 50 micrometers/s at 50 mM KCl, the highest velocities reported for skeletal myosin in usual in vitro assay systems. Both the sliding velocity and force increased by an order of magnitude when heated from 18 degrees C to 40-45 degrees C. Temperature-pulse microscopy is expected to be useful for studies of biomolecules and cells requiring temporal and/or spatial thermal modulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.