ArticleProceedings of the National Academy of Sciences of the United States of America1999
Phosphorylated tau can promote tubulin assembly.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Quantitative chemoproteomics reveals dopamine's protective modification of Tau.Nature chemical biology · 2025Article
- Tau Oligomers in Alzheimer's Disease: Modulation Effect of Osmolytes on Amplified Brain-Derived Tau Oligomers.ACS chemical neuroscience · 2025Article
- Effect of Natural Osmolytes on Recombinant Tau Monomer: Propensity of Oligomerization and Aggregation.ACS chemical neuroscience · 2024Article
- Microbial-derived metabolites as a risk factor of age-related cognitive decline and dementia.Molecular neurodegeneration · 2022Review
- Implication of Trimethylamine N-Oxide (TMAO) in Disease: Potential Biomarker or New Therapeutic Target.Nutrients · 2018Review
- Prion-Like Propagation of Post-Translationally Modified Tau in Alzheimer's Disease: A Hypothesis.Journal of molecular neuroscience : MN · 2018Review
- Imbalanced Expression of Tau and Tubulin Induces Neuronal Dysfunction inFrontiers in neuroscience · 2018Article
- Absence of a Role for Phosphorylation in the Tau Pathology of Alzheimer's Disease.Biomolecules · 2016Article
- Behind the curtain of tauopathy: a show of multiple players orchestrating tau toxicity.Cellular and molecular life sciences : CMLS · 2016Review
- Global analysis of phosphorylation of tau by the checkpoint kinases Chk1 and Chk2 in vitro.Journal of proteome research · 2013Article
- Inorganic lead (Pb)- and mercury (Hg)-induced neuronal cell death involves cytoskeletal reorganization.Laboratory animal research · 2011Article
- Mechanochemical modeling of dynamic microtubule growth involving sheet-to-tube transition.PloS one · 2011Article
- Role of APC and DNA mismatch repair genes in the development of colorectal cancers.Molecular cancer · 2003Review
- Tau function and dysfunction in neurons: its role in neurodegenerative disorders.Molecular neurobiology · 2002Review
- FEZ1/LZTS1 gene at 8p22 suppresses cancer cell growth and regulates mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Article
- Pregnenolone binds to microtubule-associated protein 2 and stimulates microtubule assembly.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorylation can affect the function of microtubule-associated protein tau. Here, the human brain tau with 441 amino acids was phosphorylated by cyclic-AMP-dependent protein kinase (PKA) or glycogen synthase kinase-3beta. PKA-phosphorylated tau (2.7 mol phosphates/mol) does not promote tubulin assembly as judged by spectrophotometric and atomic force microscopy measurements, unless trimethylamine N-oxide (TMAO), a natural occurring osmolyte, is included in these assays. TMAO is also found to promote tubulin assembly of glycogen synthase kinase-3beta-phosphorylated tau (1.6 mol phosphates/mol). TMAO does not act by causing a chemical dephosphorylation of phosphorylated tau, but it acts to overcome the functional deficit caused by phosphorylation. PKA-phosphorylated tau binds to tubulin in the presence of TMAO and lowers the critical concentration of tubulin needed for assembly. From these data, we conclude that PKA-phosphorylated tau retains the ability to bind tubulin and promote tubulin assembly. TMAO is required, however, to sensitize the reaction. Possible uses of TMAO in relation to studies of tubulin assembly in vitro, in intact cells, and in relation to Alzheimer's disease are presented in this report.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.