ArticleThe Plant cell1999
Polyadenylation occurs at multiple sites in maize mitochondrial cox2 mRNA and is independent of editing status.
Article in The Plant cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Selection of reference genes for normalization of mitochondrial gene expression by qRT-PCR in different potato tissues and during anther development.BMC plant biology · 2025Article
- Mitochondrial mRNA Processing in the Chlorophyte AlgaPlants (Basel, Switzerland) · 2021Article
- RNA polyadenylation and its consequences in prokaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Nontemplated terminal nucleotidyltransferase activity of double-stranded RNA bacteriophage phi6 RNA-dependent RNA polymerase.Journal of virology · 2008Article
- Heat stress enhances the accumulation of polyadenylated mitochondrial transcripts in Arabidopsis thaliana.PloS one · 2008Article
- A Honglian CMS line of rice displays aberrant F0 of F0F1-ATPase.Plant cell reports · 2007Article
- Mapping of mitochondrial mRNA termini in Arabidopsis thaliana: t-elements contribute to 5' and 3' end formation.Nucleic acids research · 2007Article
- A role for mitochondria in the establishment and maintenance of the maize root quiescent center.Plant physiology · 2006Article
- Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants.Plant physiology · 2005Article
- UTP-dependent turnover of Trypanosoma brucei mitochondrial mRNA requires UTP polymerization and involves the RET1 TUTase.RNA (New York, N.Y.) · 2005Article
- Distant sequences determine 5' end formation of cox3 transcripts in Arabidopsis thaliana ecotype C24.Nucleic acids research · 2005Article
- Mitochondrial cytochrome c oxidase and succinate dehydrogenase complexes contain plant specific subunits.Plant molecular biology · 2004Article
- Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase.Nucleic acids research · 2004Article
- AtmtPNPase is required for multiple aspects of the 18S rRNA metabolism in Arabidopsis thaliana mitochondria.Nucleic acids research · 2004Article
- Surprising features of plastid ndhD transcripts: addition of non-encoded nucleotides and polysome association of mRNAs with an unedited start codon.Nucleic acids research · 2004Article
- Domain analysis of the chloroplast polynucleotide phosphorylase reveals discrete functions in RNA degradation, polyadenylation, and sequence homology with exosome proteins.The Plant cell · 2003Article
- RT-PCR analysis of 5' to 3'-end-ligated mRNAs identifies the extremities of cox2 transcripts in pea mitochondria.Nucleic acids research · 2002Article
- Chloroplast PNPase exists as a homo-multimer enzyme complex that is distinct from the Escherichia coli degradosome.RNA (New York, N.Y.) · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Polyadenylation of nucleus-encoded transcripts has a well-defined role in gene expression. The extent and function of polyadenylation in organelles and prokaryotic systems, however, are less well documented. Recent reports of polyadenylation-mediated RNA destabilization in Escherichia coli and in vascular plant chloroplasts prompted us to look for polyadenylation in plant mitochondria. Here, we report the use of reverse transcription-polymerase chain reaction to map multiple polyadenylate addition sites in maize mitochondrial cox2 transcripts. The lack of sequence conservation surrounding these sites suggests that polyadenylation may occur at many 3' termini created by endoribonucleolytic and/or exoribonucleolytic activities, including those activities involved in 3' end maturation. Endogenous transcripts could be efficiently polyadenylated in vitro by using maize mitochondrial lysates with an activity that added AMP more efficiently than GMP. Polyadenylated substrates were tested for stability in maize mitochondrial S100 extracts, and we found that, compared with nonpolyadenylated RNAs, the polyadenylated substrates were less stable. Taken together with the low abundance of polyadenylated RNAs in maize mitochondria, our results are consistent with a degradation-related process. The fact that polyadenylation does not dramatically destabilize plant mitochondrial transcripts, at least in vitro, is in agreement with results obtained for animal mitochondria but differs from those obtained for chloroplasts and E. coli. Because fully edited, partially edited, and unedited transcripts were found among the cloned polyadenylated cox2 cDNAs, we conclude that RNA editing and polyadenylation are independent processes in maize mitochondria.
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