ArticleThe Plant cell1999
Multiubiquitin chain binding subunit MCB1 (RPN10) of the 26S proteasome is essential for developmental progression in Physcomitrella patens.
Article in The Plant cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- A FLOE-related protein regulates the two-dimensional to three-dimensional growth transition in the moss Physcomitrium patens.Development (Cambridge, England) · 2025Article
- Ubiquitin receptors play redundant roles in the proteasomal degradation of the p53 repressor MDM2.FEBS letters · 2022Article
- The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 inGenes · 2022Article
- RPN10: A Case Study for Ubiquitin Binding Proteins and More.The Plant cell · 2019Article
- Genetic Regulation of the 2D to 3D Growth Transition in the Moss Physcomitrella patens.Current biology : CB · 2018Article
- Polyubiquitin-Photoactivatable Crosslinking Reagents for Mapping Ubiquitin Interactome Identify Rpn1 as a Proteasome Ubiquitin-Associating Subunit.Cell chemical biology · 2017Article
- In vivo relevance of substrate recognition function of major Arabidopsis ubiquitin receptors.Plant signaling & behavior · 2012Article
- Developing a model of plant hormone interactions.Plant signaling & behavior · 2011Review
- Proteasome regulation, plant growth and stress tolerance.Plant signaling & behavior · 2009Review
- The RPN5 subunit of the 26s proteasome is essential for gametogenesis, sporophyte development, and complex assembly in Arabidopsis.The Plant cell · 2009Article
- Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development.Molecular and cellular biology · 2007Article
- The RPN1 subunit of the 26S proteasome in Arabidopsis is essential for embryogenesis.The Plant cell · 2005Article
- Tripeptidyl peptidase II. An oligomeric protease complex from Arabidopsis.Plant physiology · 2005Article
- Physcomitrella patens and Ceratodon purpureus, mosses as model organisms in photosynthesis studies.Photosynthesis research · 2005Article
- Characterization and functional identification of a novel plant 4,5-extradiol dioxygenase involved in betalain pigment biosynthesis in Portulaca grandiflora.Plant physiology · 2004Article
- An improved and highly standardised transformation procedure allows efficient production of single and multiple targeted gene-knockouts in a moss, Physcomitrella patens.Current genetics · 2004Article
- A tool for understanding homologous recombination in plants.Plant cell reports · 2003Review
- Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: implication for land plant evolution.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling.The Plant cell · 2003Article
- High frequency of phenotypic deviations in Physcomitrella patens plants transformed with a gene-disruption library.BMC plant biology · 2002Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 26S proteasome, a multisubunit complex, is the primary protease of the ubiquitin-mediated proteolytic system in eukaryotes. We have recently characterized MCB1 (RPN10), a subunit of the 26S complex that has affinity for multiubiquitin chains in vitro and as a result may function as a receptor for ubiquitinated substrates. To define the role of MCB1 further, we analyzed its function in Physcomitrella patens by generating MCB1 gene disruptions using homologous recombination. PpMCB1, which is 50 to 75% similar to orthologs from other eukaryotes, is present in the 26S proteasome complex and has a similar affinity for multiubiquitin chains, using a conserved hydrophobic domain within the C-terminal half of the polypeptide. Unlike yeast Deltamcb1 strains, which grow normally, P. patens Deltamcb1 strains are viable but are under developmental arrest, generating abnormal caulonema that are unable to form buds and gametophores. Treatment with auxin and cytokinin restored bud formation and subsequent partial development of gametophores. Complementation of a Deltamcb1 strain with mutated versions of PpMCB1 revealed that the multiubiquitin chain binding site is not essential for the wild-type phenotype. These results show that MCB1 has an important function in the 26S proteasome of higher order eukaryotes in addition to its ability to bind multiubiquitin chains, and they provide further support for a role of the ubiquitin/26S proteasome proteolytic pathway in plant developmental processes triggered by hormones.
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