ArticleThe Journal of experimental medicine1999
Transport of bacterial lipopolysaccharide to the golgi apparatus.
Article in The Journal of experimental medicine, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 120 citations in OpenAlex.
- Article
- Irgm2 and Gate-16 cooperatively dampen Gram-negative bacteria-induced caspase-11 response.EMBO reports · 2020Article
- Role of the Golgi Apparatus in the Blood-Brain Barrier: Golgi Protection May Be a Targeted Therapy for Neurological Diseases.Molecular neurobiology · 2018Review
- Article
- Biphalin, a Dimeric Enkephalin, Alleviates LPS-Induced Activation in Rat Primary Microglial Cultures in Opioid Receptor-Dependent and Receptor-Independent Manners.Neural plasticity · 2017Article
- Morphine enhances IL-1β release through toll-like receptor 4-mediated endocytic pathway in microglia.Purinergic signalling · 2016Article
- Blood-Borne Lipopolysaccharide Is Rapidly Eliminated by Liver Sinusoidal Endothelial Cells via High-Density Lipoprotein.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- CD14 Is a Co-Receptor for TLR4 in the S100A9-Induced Pro-Inflammatory Response in Monocytes.PloS one · 2016Article
- Dose response of endotoxin on hepatocyte and muscle mitochondrial respiration in vitro.BioMed research international · 2015Article
- Differential role for p120-catenin in regulation of TLR4 signaling in macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Modulating LPS signal transduction at the LPS receptor complex with synthetic Lipid A analogues.Advances in carbohydrate chemistry and biochemistry · 2014Review
- Lung cell-specific modulation of LPS-induced TLR4 receptor and adaptor localization.Communicative & integrative biology · 2014Review
- Glia maturation factor-γ negatively modulates TLR4 signaling by facilitating TLR4 endocytic trafficking in macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Surfactant protein-A modulates LPS-induced TLR4 localization and signaling via β-arrestin 2.PloS one · 2013Article
- Soluble glucan is internalized and trafficked to the Golgi apparatus in macrophages via a clathrin-mediated, lipid raft-regulated mechanism.The Journal of pharmacology and experimental therapeutics · 2012Article
- Direct engagement of TLR4 in invariant NKT cells regulates immune diseases by differential IL-4 and IFN-γ production in mice.PloS one · 2012Article
- Down-regulation of endothelial TLR4 signalling after apo A-I gene transfer contributes to improved survival in an experimental model of lipopolysaccharide-induced inflammation.Journal of molecular medicine (Berlin, Germany) · 2011Article
- Lipopolysaccharide inhibits the channel activity of the P2X7 receptor.Mediators of inflammation · 2011Article
- Rab7b and receptors trafficking.Communicative & integrative biology · 2010Article
- Toll-like receptor 4 modulation as a strategy to treat sepsis.Mediators of inflammation · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Addition of lipopolysaccharide (LPS) to cells in the form of LPS-soluble (s)CD14 complexes induces strong cellular responses. During this process, LPS is delivered from sCD14 to the plasma membrane, and the cell-associated LPS is then rapidly transported to an intracellular site. This transport appears to be important for certain cellular responses to LPS, as drugs that block transport also inhibit signaling and cells from LPS-hyporesponsive C3H/HeJ mice fail to exhibit this transport. To identify the intracellular destination of fluorescently labeled LPS after its delivery from sCD14 into cells, we have made simultaneous observations of different organelles using fluorescent vital dyes or probes. Endosomes, lysosomes, the endoplasmic reticulum, and the Golgi apparatus were labeled using Texas red (TR)-dextran, LysoTrackertrade mark Red DND-99, DiOC6(3), and boron dipyrromethane (BODIPY)-ceramide, respectively. After 30 min, LPS did not colocalize with endosomes, lysosomes, or endoplasmic reticulum in polymorphonuclear leukocytes, although some LPS-positive vesicles overlapped with the endosomal marker, fluorescent dextran. On the other hand, LPS did appear to colocalize with two markers of the Golgi apparatus, BODIPY-ceramide and TRITC (tetramethylrhodamine isothiocyanate)-labeled cholera toxin B subunit. We further confirmed the localization of LPS in the Golgi apparatus using an epithelial cell line, HeLa, which responds to LPS-sCD14 complexes in a CD14-dependent fashion: BODIPY-LPS was internalized and colocalized with fluorescently labeled Golgi apparatus probes in live HeLa cells. Morphological disruption of the Golgi apparatus in brefeldin A-treated HeLa cells caused intracellular redistribution of fluorescent LPS. These results are consistent with the Golgi apparatus being the primary delivery site of monomeric LPS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.