ArticleThe Journal of experimental medicine1999
Internalization of monomeric lipopolysaccharide occurs after transfer out of cell surface CD14.
Article in The Journal of experimental medicine, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 76 citations in OpenAlex.
- Macrophage Proteomic Profiling Reveals Divergent TLR4-Dependent and -Independent Responses to KdoLife (Basel, Switzerland) · 2026Article
- The impact of glycosylation on the structure, function, and interactions of CD14.Glycobiology · 2024Article
- Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals.Scientific reports · 2022Article
- Article
- Assessment ofVeterinary medicine international · 2017Article
- CD36 Differently Regulates Macrophage Responses to Smooth and Rough Lipopolysaccharide.PloS one · 2016Article
- Dose response of endotoxin on hepatocyte and muscle mitochondrial respiration in vitro.BioMed research international · 2015Article
- Modulating LPS signal transduction at the LPS receptor complex with synthetic Lipid A analogues.Advances in carbohydrate chemistry and biochemistry · 2014Review
- Filamentous morphology of bacteria delays the timing of phagosome morphogenesis in macrophages.The Journal of cell biology · 2013Article
- Consequences of alteration in leucine zipper sequence of melittin in its neutralization of lipopolysaccharide-induced proinflammatory response in macrophage cells and interaction with lipopolysaccharide.The Journal of biological chemistry · 2012Article
- TLR4 signalling in pulmonary stromal cells is critical for inflammation and immunity in the airways.Respiratory research · 2011Review
- Deciphering the complexity of Toll-like receptor signaling.Cellular and molecular life sciences : CMLS · 2010Review
- Immune physiology in tissue regeneration and aging, tumor growth, and regenerative medicine.Aging · 2009Review
- Intracellular recognition of lipopolysaccharide by toll-like receptor 4 in intestinal epithelial cells.The Journal of experimental medicine · 2003Article
- Toll-like receptor 4 resides in the Golgi apparatus and colocalizes with internalized lipopolysaccharide in intestinal epithelial cells.The Journal of experimental medicine · 2002Article
- Gram-positive and gram-negative bacteria do not trigger monocytic cytokine production through similar intracellular pathways.Infection and immunity · 2001Article
- Transport of bacterial lipopolysaccharide to the golgi apparatus.The Journal of experimental medicine · 1999Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipopolysaccharide (LPS) fluorescently labeled with boron dipyrromethane (BODIPY) first binds to the plasma membrane of CD14-expressing cells and is subsequently internalized. Intracellular LPS appears in small vesicles near the cell surface and later in larger, punctate structures identified as the Golgi apparatus. To determine if membrane (m)CD14 directs the movement of LPS to the Golgi apparatus, an mCD14 chimera containing enhanced green fluorescent protein (mCD14-EGFP) was used to follow trafficking of mCD14 and BODIPY-LPS in stable transfectants. The chimera was expressed strongly on the cell surface and also in a Golgi complex-like structure. mCD14-EGFP was functional in mediating binding of and responses to LPS. BODIPY-LPS presented to the transfectants as complexes with soluble CD14 first colocalized with mCD14-EGFP on the cell surface. However, within 5-10 min, the BODIPY-LPS distributed to intracellular vesicles that did not contain mCD14-EGFP, indicating that mCD14 did not accompany LPS during endocytic movement. These results suggest that monomeric LPS is transferred out of mCD14 at the plasma membrane and traffics within the cell independently of mCD14. In contrast, aggregates of LPS were internalized in association with mCD14, suggesting that LPS clearance occurs via a pathway distinct from that which leads to signaling via monomeric LPS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.