ArticleJournal of clinical microbiology1999
Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Using a bayesian latent class model to evaluate the utility of investigating persons with negative polymerase chain reaction results for pertussis.American journal of epidemiology · 2013Pooled it
- How to identify respiratory pathogens in primary health care - a review on the benefits, prospects and pitfalls in using point of care tests.Infection · 2025Review
- Laboratory Performance and Clinical Correlation of Real-time Polymerase Chain Reaction as a Diagnostic Test forOman medical journal · 2022Article
- Spatio-temporal comparison of pertussis outbreaks in Olmsted County, Minnesota, 2004-2005 and 2012: a population-based study.BMJ open · 2019Article
- Emergence of Bordetella holmesii as a Causative Agent of Whooping Cough, Barcelona, Spain.Emerging infectious diseases · 2017Article
- The pertussis enigma: reconciling epidemiology, immunology and evolution.Proceedings. Biological sciences · 2016Review
- Protecting Newborns Against Pertussis: Treatment and Prevention Strategies.Paediatric drugs · 2015Review
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Diagnosis of pertussis using nasopharyngeal IgA and polymerase chain reaction in specimens from outpatients in Australia.European journal of microbiology & immunology · 2014Article
- Epidemiological aspects of pertussis among adults and adolescents in a Korean outpatient setting: a multicenter, PCR-based study.Journal of Korean medical science · 2014Article
- Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.Iranian journal of microbiology · 2014Article
- Estimating the burden of pertussis in young children on hospitals and emergency departments: a study using linked routinely collected data.Epidemiology and infection · 2014Article
- Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.Iranian journal of microbiology · 2013Article
- A Multicenter Study of Pertussis Infection in Adults with Coughing in Korea: PCR-Based Study.Tuberculosis and respiratory diseases · 2012Article
- Towards improved accuracy of Bordetella pertussis nucleic acid amplification tests.Journal of clinical microbiology · 2012Review
- The laboratory diagnosis of Bordetella pertussis infection: a comparison of semi-nested PCR and real-time PCR with culture.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Increased risk of pertussis in patients with asthma.The Journal of allergy and clinical immunology · 2012Article
- Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.Journal of clinical microbiology · 2008Article
- Utility of composite reference standards and latent class analysis in evaluating the clinical accuracy of diagnostic tests for pertussis.Clinical and vaccine immunology : CVI · 2008Article
- Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis.European journal of pediatrics · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We prospectively compared the performance of culture, direct fluorescent-antibody testing (DFA), and an in-house-developed PCR test targeting the repeated insertion sequence IS481 for the detection of Bordetella pertussis in nasopharyngeal swab specimens. We tested 319 consecutive paired specimens on which all three tests were performed. A total of 59 specimens were positive by one or more tests. Of these, 5 were positive by all three tests, 2 were positive by culture and PCR, 16 were positive by PCR and DFA, 28 were positive by PCR only, and 8 were positive by DFA only. Any specimen positive by culture was considered to be a true positive, as were specimens positive by both PCR and DFA. Specimens positive only by PCR or DFA were considered discrepant, and their status was resolved by review of patient histories. Patients with symptoms meeting the Centers for Disease Control and Prevention clinical case definition for pertussis and who had a specimen positive by PCR or DFA were considered to have true B. pertussis infections. Of the 28 patients positive by PCR only, 20 met the clinical case definition for pertussis, while 3 of the 8 patients positive by DFA only met the clinical case definition. After resolution of the status of discrepant specimens, the sensitivity, specificity, positive predictive value, and negative predictive value were 15.2, 100, 100, and 87.5%, respectively, for culture; 93.5, 97.1, 84.3, and 98.9%, respectively, for PCR; and 52.2, 98.2, 82.8, and 92.4%, respectively, for DFA. The actual positive predictive value of PCR was probably greater, as several PCR-positive patients who did not meet the clinical case definition had symptoms consistent with typical or atypical pertussis. PCR is a sensitive and specific method for the detection of B. pertussis.
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