ArticleJournal of clinical microbiology1999
Use of molecular assays in diagnosis and monitoring of cytomegalovirus disease following renal transplantation.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Comparison of quantitative cytomegalovirus (CMV) PCR in plasma and CMV antigenemia assay: clinical utility of the prototype AMPLICOR CMV MONITOR test in transplant recipients.Journal of clinical microbiology · 2000Trial
- Cytomegalovirus pneumonia in a background of central nervous system tuberculosis.Respirology case reports · 2022Article
- High Human Cytomegalovirus IgG Level is Associated with Increased Incidence of Diabetic Atherosclerosis in Type 2 Diabetes Mellitus Patients.Medical science monitor : international medical journal of experimental and clinical research · 2015Article
- Detection of cytomegalovirus DNA in plasma as an adjunct diagnostic for gastrointestinal tract disease in kidney and liver transplant recipients.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2013Article
- Time profile of viral DNA in aqueous humor samples of patients treated for varicella-zoster virus acute retinal necrosis by use of quantitative real-time PCR.Journal of clinical microbiology · 2013Article
- Multi-Site PCR-based CMV viral load assessment-assays demonstrate linearity and precision, but lack numeric standardization: a report of the association for molecular pathology.The Journal of molecular diagnostics : JMD · 2009Article
- Monitoring of cytomegalovirus reactivation in bone marrow transplant recipients by real-time PCR.Pathology oncology research : POR · 2008Article
- Evaluation of the COBAS Amplicor HCMV Monitor for early detection and monitoring of human cytomegalovirus infection after allogeneic stem cell transplantation.Bone marrow transplantation · 2006Article
- Comparison of molecular tests for detection and quantification of cell-associated cytomegalovirus DNA.Journal of clinical microbiology · 2003Article
- Comparison of LightCycler-based PCR, COBAS amplicor CMV monitor, and pp65 antigenemia assays for quantitative measurement of cytomegalovirus viral load in peripheral blood specimens from patients after solid organ transplantation.Journal of clinical microbiology · 2003Article
- Accuracy of the serological ELISA test compared with the polymerase chain reaction for the diagnosis of cytomegalovirus infection in pregnancy.Sao Paulo medical journal = Revista paulista de medicina · 2003Article
- Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients.Journal of clinical microbiology · 2003Article
- Normalized quantification of human cytomegalovirus DNA by competitive real-time PCR on the LightCycler instrument.Journal of clinical microbiology · 2002Article
- Monitoring of viral load by quantitative plasma PCR during active cytomegalovirus infection of individual liver transplant patients.Journal of clinical microbiology · 2002Article
- Distinguishing cytomegalovirus (CMV) infection and disease with CMV nucleic acid assays.Journal of clinical microbiology · 2002Article
- Evaluation of the Murex CMV DNA Hybrid Capture assay (version 2.0) for early diagnosis of cytomegalovirus infection in recipients of an allogeneic stem cell transplant.Bone marrow transplantation · 2001Article
- LightCycler-based quantitative PCR for detection of cytomegalovirus in blood, urine, and respiratory samples.Journal of clinical microbiology · 2001Article
- CMV infection of liver transplant recipients: comparison of antigenemia and molecular biology assays.BMC infectious diseases · 2001Article
- Predictive value of quantitative PCR-based viral burden analysis for eight human herpesviruses in pediatric solid organ transplant patients.The Journal of molecular diagnostics : JMD · 2000Article
- Detection of cytomegalovirus DNA in human specimens by LightCycler PCR.Journal of clinical microbiology · 2000Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared two commercial molecular assays (the Murex Hybrid Capture CMV DNA assay [HCA], version 2, and the Roche Amplicor plasma PCR assay) with a standard shell vial assay in detecting and predicting cytomegalovirus (CMV) disease in a group of renal transplant patients and assessed the role of viral load measurements (using the HCA) in their management. The sensitivity of the HCA and Amplicor assay in terms of disease detection was 100%, compared to 71% for the shell vial assay. Both the HCA and the PCR assay detected all cases of disease, at medians of 11 and 12.5 days before the onset of symptoms, respectively. Significantly higher viral loads were detected in those patients with symptoms (7.9 x 10(5) copies/ml) than in patients without symptoms (7.9 x 10(4) copies/ml; P < 0.0001). There was also a trend towards higher viral loads in those patients with primary infections (7.8 x 10(5) copies/ml) than in those patients with reactivations of CMV disease or reinfections. Successful treatment with ganciclovir was associated with a >90% reduction in viral load. Both of these new assays are sensitive and easy to use. A comparison of accurate quantitation is also useful in monitoring responses to antiviral therapy.
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