ArticleThe Journal of clinical investigation1999
Rapid downregulation of rat renal Na/P(i) cotransporter in response to parathyroid hormone involves microtubule rearrangement.
Article in The Journal of clinical investigation, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 120 citations in OpenAlex.
- The Intricacies of Renal Phosphate Reabsorption-An Overview.International journal of molecular sciences · 2024Review
- Osteomalacia in Adults: A Practical Insight for Clinicians.Journal of clinical medicine · 2023Review
- Mechanisms of phosphate transport.Nature reviews. Nephrology · 2019Review
- Visualizing the regulation of SLC34 proteins at the apical membrane.Pflugers Archiv : European journal of physiology · 2019Review
- NaPi-IIa interacting partners and their (un)known functional roles.Pflugers Archiv : European journal of physiology · 2019Review
- Phosphate and FGF-23 homeostasis after kidney transplantation.Nature reviews. Nephrology · 2015Review
- Renal phosphate wasting in the absence of adenylyl cyclase 6.Journal of the American Society of Nephrology : JASN · 2014Article
- Exome sequencing identifies a novel homozygous mutation in the phosphate transporter SLC34A1 in hypophosphatemia and nephrocalcinosis.The Journal of clinical endocrinology and metabolism · 2014Article
- Activation of a non-cAMP/PKA signaling pathway downstream of the PTH/PTHrP receptor is essential for a sustained hypophosphatemic response to PTH infusion in male mice.Endocrinology · 2013Article
- Inorganic phosphate modulates the expression of the NaPi-2a transporter in the trans-Golgi network and the interaction with PIST in the proximal tubule.BioMed research international · 2013Article
- Vitamin D and the kidney.Archives of biochemistry and biophysics · 2012Review
- Differential modulation of the molecular dynamics of the type IIa and IIc sodium phosphate cotransporters by parathyroid hormone.American journal of physiology. Cell physiology · 2011Article
- Transgenic overexpression of the extra-large Gsα variant XLαs enhances Gsα-mediated responses in the mouse renal proximal tubule in vivo.Endocrinology · 2011Article
- Acute parathyroid hormone differentially regulates renal brush border membrane phosphate cotransporters.Pflugers Archiv : European journal of physiology · 2010Article
- PTH-induced internalization of apical membrane NaPi2a: role of actin and myosin VI.American journal of physiology. Cell physiology · 2009Article
- Mechanisms of renal phosphate loss in liver resection-associated hypophosphatemia.Annals of surgery · 2009Article
- Kidney and phosphate metabolism.Electrolyte & blood pressure : E & BP · 2008Review
- Effects of prolonged high phosphorus diet on phosphorus and calcium balance in rats.Journal of clinical biochemistry and nutrition · 2007Article
- Phosphatonin washout in Hyp mice proximal tubules: evidence for posttranscriptional regulation.American journal of physiology. Renal physiology · 2005Article
- Regulation of NaPi-IIa mRNA and transporter protein in chronic renal failure: role of parathyroid hormone (PTH) and dietary phosphate (Pi).Pflugers Archiv : European journal of physiology · 2004Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Renal proximal tubule cells express in their apical brush border membrane (BBM) a Na/P(i) cotransporter type IIa that is rapidly downregulated in response to parathyroid hormone (PTH). We used the rat renal Na/P(i) cotransporter type IIa (NaPi-2) as an in vivo model to assess early cellular events in the rapid downregulation of this transporter. When rats were treated with PTH for 15 minutes, NaPi-2 abundance in the BBM was decreased. In parallel, transporter accumulated in intracellular vesicles. Concomitantly, microtubules (MTs) were found to form dense bundles of apical-to-basal orientation. After 60 minutes of PTH action, the cells were vastly depleted of NaPi-2, whereas their microtubular cytoskeleton had returned to its normal appearance. Prevention of MT rearrangement by taxol resulted in accumulation of NaPi-2 in the subapical cell portion after 15 minutes and a strong delay in depletion of intracellular transporter after 60 minutes of PTH action. Furthermore, the subapical accumulation of NaPi-2 was associated with the expansion of dense apical tubules of the subapical endocytic apparatus (SEA). Depolymerization of MTs by colchicine likewise caused a retardation of intracellular NaPi-2 depletion. These results suggest that NaPi-2 is downregulated in response to PTH through a rapid endocytic process in 2 separate steps: (a) internalization of the transporter into the SEA, and (b) its delivery to degradative organelles by a trafficking mechanism whose efficiency depends on a taxol-sensitive rearrangement of MTs.
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