ArticleGenome research1999
Physical map and organization of chromosome 7 in the rice blast fungus, Magnaporthe grisea.
Article in Genome research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- The role of transposable element clusters in genome evolution and loss of synteny in the rice blast fungus Magnaporthe oryzae.Genome biology · 2006Article
- The rice blast pathosystem as a case study for the development of new tools and raw materials for genome analysis of fungal plant pathogens.The New phytologist · 2003Review
- Internet Contig Explorer (iCE)--a tool for visualizing clone fingerprint maps.Genome research · 2003Article
- Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea.The EMBO journal · 2003Article
- Generalized gap model for bacterial artificial chromosome clone fingerprint mapping and shotgun sequencing.Genome research · 2002Article
- AFLP-derived SCARs facilitate construction of a 1.1 Mb sequence-ready map of a region that spans the Vf locus in the apple genome.Plant molecular biology · 2002Article
- MagnaportheDB: a federated solution for integrating physical and genetic map data with BAC end derived sequences for the rice blast fungus Magnaporthe grisea.Nucleic acids research · 2002Article
- Bacterial artificial chromosome-based physical map of the rice genome constructed by restriction fingerprint analysis.Genetics · 2001Article
- Agricultural microbes genome 2: first glimpses into the genomes of plant-associated microbes.The Plant cell · 2001Article
- Contigs built with fingerprints, markers, and FPC V4.7.Genome research · 2000Article
- Article
- A high-throughput AFLP-based method for constructing integrated genetic and physical maps: progress toward a sorghum genome map.Genome research · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rice blast fungus Magnaporthe grisea is a highly destructive plant pathogen and one of the most important for studying various aspects of host-plant interactions. It has been widely adopted as a model organism because it is ideally suited for genetic and biological studies. To facilitate map-based cloning, chromosome walking, and genome organization studies of M. grisea, a complete physical map of chromosome 7 was constructed using a large-insert (130 kb) bacterial artificial chromosome (BAC) library. Using 147 chromosome 7-specific single-copy BAC clones and 20 RFLP markers on chromosome 7, 625 BAC clones were identified by hybridization. BAC clones were digested with HindIII, and fragments were size separated on analytical agarose gels to create DNA fingerprints. Hybridization contigs were constructed using a random cost algorithm, whereas fingerprinting contigs were constructed using the software package FPC. Results from both methods were generally in agreement, but numerous anomalies were observed. The combined data produced five robust anchored contigs after gap closure by chromosomal walking. The genetic and physical maps agreed closely. The final physical map was estimated to cover >95% of the 4.2 Mb of chromosome 7. Based on the contig maps, a minimum BAC tile containing 42 BAC clones was created, and organization of repetitive elements and expressed genes of the chromosome was investigated.
Indexed as
Identifiers
10447509PMC310806W1950961684What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.