ArticleRNA (New York, N.Y.)1999
In vitro selection of RNAs with increased tertiary structure stability.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- In Crystallo Selection to Establish New RNA Crystal Contacts.Structure (London, England : 1993) · 2018Article
- Molecular Mechanism for Folding Cooperativity of Functional RNAs in Living Organisms.Biochemistry · 2018Article
- An in vitro-selected RNA receptor for the GAAC loop: modular receptor for non-GNRA-type tetraloop.Nucleic acids research · 2013Article
- Metal-ion rescue revisited: biochemical detection of site-bound metal ions important for RNA folding.RNA (New York, N.Y.) · 2012Article
- Removal of covalent heterogeneity reveals simple folding behavior for P4-P6 RNA.The Journal of biological chemistry · 2011Article
- Generation and development of RNA ligase ribozymes with modular architecture through "design and selection".Molecules (Basel, Switzerland) · 2010Review
- Identification and characterization of anion binding sites in RNA.RNA (New York, N.Y.) · 2010Article
- Improving small-angle X-ray scattering data for structural analyses of the RNA world.RNA (New York, N.Y.) · 2010Article
- Selective stabilization of natively folded RNA structure by DNA constraints.Journal of the American Chemical Society · 2008Article
- Toward predicting self-splicing and protein-facilitated splicing of group I introns.RNA (New York, N.Y.) · 2008Article
- Selection of a novel class of RNA-RNA interaction motifs based on the ligase ribozyme with defined modular architecture.Nucleic acids research · 2008Article
- Comprehensive features of natural and in vitro selected GNRA tetraloop-binding receptors.Nucleic acids research · 2008Article
- Local RNA structural changes induced by crystallization are revealed by SHAPE.RNA (New York, N.Y.) · 2007Article
- Topological rearrangement yields structural stabilization and interhelical distance constraints in the Kin.46 self-phosphorylating ribozyme.RNA (New York, N.Y.) · 2006Article
- In vitro selection of high temperature Zn(2+)-dependent DNAzymes.Journal of molecular evolution · 2005Article
- Tris-borate is a poor counterion for RNA: a cautionary tale for RNA folding studies.Nucleic acids research · 2004Article
- Generation of a catalytic module on a self-folding RNA.RNA (New York, N.Y.) · 2004Article
- An early transition state for folding of the P4-P6 RNA domain.RNA (New York, N.Y.) · 2001Article
- Quantifying the energetic interplay of RNA tertiary and secondary structure interactions.RNA (New York, N.Y.) · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
An in vitro selection system was devised to select RNAs based on their tertiary structural stability, independent of RNA activity. Selection studies were conducted on the P4-P6 domain from the Tetrahymena thermophila group I intron, an autonomous self-folding unit that contains several important tertiary folding motifs including the tetraloop receptor and the A-rich bulge. Partially randomized P4-P6 molecules were selected based on their ability to fold into compact structures using native gel electrophoresis in the presence of decreasing concentrations of MgCl2. After 10 rounds of the selection process, a number of sequence alterations were identified that stabilized the P4-P6 RNA. One of these, a single base deletion of C209 within the P4 helix, significantly stabilized the P4-P6 molecule and would not have been identified by an activity-based selection because of its essential role for ribozyme function. Additionally, the sequence analysis provided evidence that stabilization of secondary structure may contribute to overall tertiary stability for RNAs. This system for probing RNA structure irrespective of RNA activity allows analysis of RNA structure/function relationships by identifying nucleotides or motifs important for folding and then comparing them with RNA sequences required for function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.