ArticleRNA (New York, N.Y.)1999
Pac1p, an RNase III homolog, is required for formation of the 3' end of U2 snRNA in Schizosaccharomyces pombe.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 33 citations in OpenAlex.
- Genetic screening for regulators of Prz1, a transcriptional factor acting downstream of calcineurin in fission yeast.The Journal of biological chemistry · 2012Article
- Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells.The EMBO journal · 2010Article
- The assembly of a spliceosomal small nuclear ribonucleoprotein particle.Nucleic acids research · 2008Review
- Cell cycle-dependent nuclear localization of yeast RNase III is required for efficient cell division.Molecular biology of the cell · 2004Article
- Functional significance of intermediate cleavages in the 3'ETS of the pre-rRNA from Schizosaccharomyces pombe.Nucleic acids research · 2003Article
- Conservation of RNase III processing pathways and specificity in hemiascomycetes.Eukaryotic cell · 2003Article
- The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.The EMBO journal · 2003Article
- Ribonuclease activity and RNA binding of recombinant human Dicer.The EMBO journal · 2002Article
- Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing.The EMBO journal · 2001Article
- RNA-mediated interaction of Cajal bodies and U2 snRNA genes.The Journal of cell biology · 2001Article
- Transcription of the Schizosaccharomyces pombe U2 gene in vivo and in vitro is directed by two essential promoter elements.Nucleic acids research · 2001Article
- Substrate recognition by a eukaryotic RNase III: the double-stranded RNA-binding domain of Rnt1p selectively binds RNA containing a 5'-AGNN-3' tetraloop.RNA (New York, N.Y.) · 2000Article
- Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The N-terminal domain that distinguishes yeast from bacterial RNase III contains a dimerization signal required for efficient double-stranded RNA cleavage.Molecular and cellular biology · 2000Article
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3 authors at 2 institutions in 1 country.
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Abstract
Like its homologs in higher eukaryotes, the U2 snRNA in Schizosaccharomyces pombe is transcribed by RNA polymerase II and is not polyadenylated. Instead, an RNA stem-loop structure located downstream of the U2 snRNA coding sequence and transcribed as part of a 3' extended precursor serves as a signal for 3'-end formation. We have identified three mutants that have temperature-sensitive defects in U2 snRNA 3'-end formation. In these mutants, the synthesis of the major snRNAs is also affected and unprocessed rRNA precursors accumulate at the restrictive temperature. Two of these mutants contain the same G-to-A transition within the pac1 gene, whereas the third contains a lesion outside the pac1 locus, indicating that at least two genes are involved. The pac1+ gene is codominant with the mutant allele and can rescue the temperature-sensitive phenotype and the defects in snRNA and rRNA synthesis, if overexpressed. In vitro, Pac1p, an RNase III homolog, can cleave a synthetic U2 precursor within the signal for 3'-end formation, generating a product that is a few nucleotides longer than mature U2 snRNA. In addition, U2 precursors are cleaved and trimmed to the mature size in extracts made from wild-type S. pombe cells. However, extracts made from pac1 mutant cells are unable to do so unless they are supplemented with purified recombinant Pac1p. Thus, the 3' end of S. pombe U2 snRNA is generated by a processing reaction that requires Pac1p and an additional component, and can be dissociated from transcription in vitro.
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