ArticleGenes & development1999
Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export.
Article in Genes & development, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 77 citations in OpenAlex.
- Npl3 is required for efficient autophagosome-vacuole fusion.Autophagy · 2026Article
- The prion-like protein kinase Sky1 is required for efficient stress granule disassembly.Nature communications · 2019Article
- tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Review
- Review
- mRNA quality control is bypassed for immediate export of stress-responsive transcripts.Nature · 2016Article
- UAP56 is a conserved crucial component of a divergent mRNA export pathway in Toxoplasma gondii.Molecular microbiology · 2016Article
- Recruitment, Duplex Unwinding and Protein-Mediated Inhibition of the Dead-Box RNA Helicase Dbp2 at Actively Transcribed Chromatin.Journal of molecular biology · 2016Article
- Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS.Nature neuroscience · 2015Article
- Postage for the messenger: designating routes for nuclear mRNA export.Trends in cell biology · 2013Review
- The yeast SR-like protein Npl3 links chromatin modification to mRNA processing.PLoS genetics · 2012Article
- Keeping mRNPs in check during assembly and nuclear export.Nature reviews. Molecular cell biology · 2011Article
- Pol II-directed short RNAs suppress the nuclear export of mRNA.Plant molecular biology · 2010Article
- The mitogen-activated protein kinase Slt2 regulates nuclear retention of non-heat shock mRNAs during heat shock-induced stress.Molecular and cellular biology · 2010Article
- Noncanonical transcript forms in yeast and their regulation during environmental stress.RNA (New York, N.Y.) · 2010Article
- Autoregulation of Npl3, a yeast SR protein, requires a novel downstream region and serine phosphorylation.Molecular and cellular biology · 2008Article
- HSF1-TPR interaction facilitates export of stress-induced HSP70 mRNA.The Journal of biological chemistry · 2007Article
- Following temperature stress, export of heat shock mRNA occurs efficiently in cells with mutations in genes normally important for mRNA export.Eukaryotic cell · 2007Article
- Analysis of a predicted nuclear localization signal: implications for the intracellular localization and function of the Saccharomyces cerevisiae RNA-binding protein Scp160.Nucleic acids research · 2007Article
- Nucleocytoplasmic shuttling of the Rpb4p and Rpb7p subunits of Saccharomyces cerevisiae RNA polymerase II by two pathways.Eukaryotic cell · 2006Article
- 3'-end formation signals modulate the association of genes with the nuclear periphery as well as mRNP dot formation.The EMBO journal · 2006Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Npl3p, the major mRNA-binding protein of the yeast Saccharomyces cerevisiae shuttles between the nucleus and the cytoplasm. A single amino acid change in the carboxyl terminus of Npl3p (E409 --> K) renders the mutant protein largely cytoplasmic because of a delay in its import into the nucleus. This import defect can be reversed by increasing the intracellular concentration of Mtr10p, the nuclear import receptor for Npl3p. Conversely, using this mutant, we show that Npl3p and mRNA export out of the nucleus is significantly slowed in cells bearing mutations in XPO1/CRM1, which encodes the export receptor for NES-containing proteins and in RAT7, which encodes an essential nucleoporin. Interestingly, following induction of stress by heat shock, high salt, or ethanol, conditions under which most mRNA export is blocked, Npl3p is still exported from the nucleus. The stress-induced export of Npl3p is independent of both the activity of Xpo1p and the continued selective export of heat-shock mRNAs that occurs following stress. UV-cross-linking experiments show that Npl3p is bound to mRNA under normal conditions, but is no longer RNA associated in stressed cells. Taken together, we suggest that the uncoupling of Npl3p and possibly other mRNA-binding proteins from mRNAs in the nucleus provides a general switch that regulates mRNA export. By this model, under normal conditions Npl3p is a major component of an export-competent RNP complex. However, under conditions of stress, Npl3p no longer associates with the export complex, rendering it export incompetent and thus nuclear.
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