ArticlePlant physiology1999
The arcelin-5 gene of Phaseolus vulgaris directs high seed-specific expression in transgenic Phaseolus acutifolius and Arabidopsis plants.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 67 citations in OpenAlex.
- Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis.Journal of experimental botany · 2019Article
- A vacuolar sorting receptor-independent sorting mechanism for storage vacuoles in soybean seeds.Scientific reports · 2018Article
- The pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in Arabidopsis thaliana plants.World journal of microbiology & biotechnology · 2016Article
- Seed-to-seed-to-seed growth and development of Arabidopsis in microgravity.Astrobiology · 2014Article
- Generation of VHH antibodies against the Arabidopsis thaliana seed storage proteins.Plant molecular biology · 2014Article
- Article
- Production of active human glucocerebrosidase in seeds of Arabidopsis thaliana complex-glycan-deficient (cgl) plants.Glycobiology · 2012Article
- Genetic analysis of morphological traits in a new, versatile, rapid-cycling Brassica rapa recombinant inbred line population.Frontiers in plant science · 2012Article
- Ectopic expression of a conifer Abscisic Acid Insensitive3 transcription factor induces high-level synthesis of recombinant human alpha-L-iduronidase in transgenic tobacco leaves.Plant molecular biology · 2007Article
- Aberrant localization and underglycosylation of highly accumulating single-chain Fv-Fc antibodies in transgenic Arabidopsis seeds.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences.Nature biotechnology · 2002Article
- Cotton alpha-globulin promoter: isolation and functional characterization in transgenic cotton, Arabidopsis, and tobacco.Transgenic research · 2002Article
- Plants as bioreactors for protein production: avoiding the problem of transgene silencing.Plant molecular biology · 2000Review
- Transgene silencing in monocots.Plant molecular biology · 2000Review
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulatory sequences of many genes encoding seed storage proteins have been used to drive seed-specific expression of a variety of proteins in transgenic plants. Because the levels at which these transgene-derived proteins accumulate are generally quite low, we investigated the utility of the arcelin-5 regulatory sequences in obtaining high seed-specific expression in transgenic plants. Arcelin-5 is an abundant seed protein found in some wild common bean (Phaseolus vulgaris L.) genotypes. Seeds of Arabidopsis and Tepary bean (Phaseolus acutifolius A. Gray) plants transformed with arcelin-5 gene constructs synthesized arcelin-5 to levels of 15% and 25% of the total protein content, respectively. To our knowledge, such high expression levels directed by a transgene have not been reported before. The transgenic plants also showed low plant-to-plant variation in arcelin expression. Complex transgene integration patterns, which often result in gene silencing effects, were not associated with reduced arcelin-5 expression. High transgene expression was the result of high mRNA steady-state levels and was restricted to seeds. This indicates that all requirements for high seed-specific expression are cis elements present in the cloned genomic arcelin-5 sequence and trans-acting factors that are available in Arabidopsis and Phaseolus spp., and thus probably in most dicotyledonous plants.
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