ArticleThe Journal of cell biology1999
Insoluble gamma-tubulin-containing structures are anchored to the apical network of intermediate filaments in polarized CACO-2 epithelial cells.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- MCC is a centrosomal protein that relocalizes to non-centrosomal apical sites during intestinal cell differentiation.Journal of cell science · 2022Article
- AMPK-dependent phosphorylation of cingulin reversibly regulates its binding to actin filaments and microtubules.Scientific reports · 2018Article
- Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Molecular biology of the cell · 2018Article
- Microtubule organization, dynamics and functions in differentiated cells.Development (Cambridge, England) · 2017Review
- Review
- Keratin 8 knockdown leads to loss of the chloride transporter DRA in the colon.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Plasticity of the brush border - the yin and yang of intestinal homeostasis.Nature reviews. Gastroenterology & hepatology · 2016Review
- Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat-Shock Protein Chaperoning and Kinase Rescue.Methods in enzymology · 2016Article
- Human intestinal epithelial cells express interleukin-10 through Toll-like receptor 4-mediated epithelial-macrophage crosstalk.Journal of innate immunity · 2015Article
- A note on protein expression changes in chicken breast muscle in response to time in transit before slaughtering.Proteome science · 2013Article
- Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.The Journal of biological chemistry · 2012Article
- Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium.Molecular biology of the cell · 2012Article
- Adult human CD133/1(+) kidney cells isolated from papilla integrate into developing kidney tubules.Biochimica et biophysica acta · 2011Article
- Widespread protein aggregation as an inherent part of aging in C. elegans.PLoS biology · 2010Article
- Rescue of atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones.Journal of cell science · 2009Article
- Anchoring of protein kinase A-regulatory subunit IIalpha to subapically positioned centrosomes mediates apical bile canalicular lumen development in response to oncostatin M but not cAMP.Molecular biology of the cell · 2007Article
- Intermediate filaments: a role in epithelial polarity.Experimental cell research · 2007Review
- GCP6 binds to intermediate filaments: a novel function of keratins in the organization of microtubules in epithelial cells.Molecular biology of the cell · 2007Article
- Intermediate filaments interact with dormant ezrin in intestinal epithelial cells.Molecular biology of the cell · 2005Article
- Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p.Molecular biology of the cell · 2005Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
We have previously shown that a thin ( approximately 1 microm) layer of intermediate filaments located beneath the apical membrane of a variety of simple epithelial cells participates in the organization of apical microfilaments and microtubules. Here, I confirmed the apical distribution of gamma-tubulin-containing structures (potential microtubule-organizing centers) in CACO-2 cells and demonstrated perfect colocalization of centrosomes and nearly 50% of noncentrosomal gamma-tubulin with apical intermediate filaments, but not with apical F-actin. Furthermore, the antisense-oligonucleotide-mediated downregulation of cytokeratin 19, using two different antisense sequences, was more efficient than anticytoskeletal agents to delocalize centrosomes. Electron microscopy colocalization suggests that binding occurs at the outer boundary of the pericentriolar material. Type I cytokeratins 18 and 19 present in these cells specifically coimmunoprecipitated in multi-protein fragments of the cytoskeleton with gamma-tubulin. The size and shape of the fragments, visualized at the EM level, indicate that physical trapping is an unlikely explanation for this result. Drastic changes in the extraction protocol did not affect coimmunoprecipitation. These results from three independent techniques, indicate that insoluble gamma-tubulin-containing structures are attached to apical intermediate filaments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.