ArticleThe Journal of cell biology1999
Three-dimensional visualization of transcription sites and their association with splicing factor-rich nuclear speckles.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
69 citing papers in PubMed.
- Nuclear speckle-associated perispeckle networks partition active chromatin into gene expression niches.Cell reports · 2026Article
- Lipidation as a post-translational code for protein liquid-liquid phase separation.Journal of lipid research · 2026Review
- Conservation in Geographical Utilization of Distinct Nuclear Chromatin Architectures in the Vertebrate Retinas: A Proposed Visual Adaptation.Molecular neurobiology · 2025Article
- Influenza A virus NS1 suppresses nuclear speckles promoted gene expression by inhibition of transcription.Npj viruses · 2025Article
- ZNF32 histidine 179 and 183 single-site and double-site mutations promote nuclear speckle formation but differentially regulate the proliferation of breast cancer cells.Frontiers in cell and developmental biology · 2025Article
- Control of protein synthesis through mRNA pseudouridylation by dyskerin.Science advances · 2023Article
- Coordination of transcription, processing, and export of highly expressed RNAs by distinct biomolecular condensates.Emerging topics in life sciences · 2020Article
- Review
- USP42 enhances homologous recombination repair by promoting R-loop resolution with a DNA-RNA helicase DHX9.Oncogenesis · 2020Article
- Probing the function of long noncoding RNAs in the nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- Chromosome territories and the global regulation of the genome.Genes, chromosomes & cancer · 2019Review
- Genome organization around nuclear speckles.Current opinion in genetics & development · 2019Review
- A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B.PLoS genetics · 2017Article
- The exon junction complex factor Y14 is dynamic in the nucleus of the beetleMolecular cytogenetics · 2017Article
- N-acetyl-D-glucosamine kinase is a component of nuclear speckles and paraspeckles.Molecules and cells · 2015Article
- PHD finger protein 2 (PHF2) represses ribosomal RNA gene transcription by antagonizing PHF finger protein 8 (PHF8) and recruiting methyltransferase SUV39H1.The Journal of biological chemistry · 2014Article
- Article
- Alterations in replication timing of cancer-related genes in malignant human breast cancer cells.Journal of cellular biochemistry · 2013Article
- The FF4 and FF5 domains of transcription elongation regulator 1 (TCERG1) target proteins to the periphery of speckles.The Journal of biological chemistry · 2012Article
- Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Transcription sites are detected by labeling nascent transcripts with BrUTP in permeabilized 3T3 mouse fibroblasts followed by laser scanning confocal microscopy. Inhibition and enzyme digestion studies confirm that the labeled sites are from RNA transcripts and that RNA polymerase I (RP I) and II (RP II) are responsible for nucleolar and extranucleolar transcription, respectively. An average of 2,000 sites are detected per nucleus with over 90% in the extranucleolar compartment where they are arranged in clusters and three-dimensional networklike arrays. The number of transcription sites, their three-dimensional organization and arrangement into functional zones (Wei et al. 1998) is strikingly maintained after extraction for nuclear matrix. Significant levels of total RP II mediated transcription sites (45%) were associated with splicing factor-rich nuclear speckles even though the speckles occupied <10% of the total extranucleolar space. Moreover, the vast majority of nuclear speckles (>90%) had moderate to high levels of associated transcription activity. Transcription sites were found along the periphery as well as inside the speckles themselves. These spatial relations were confirmed in optical sections through individual speckles and after in vivo labeling of nascent transcripts. Our results demonstrate that nuclear speckles and their surrounding regions are major sites of RP II-mediated transcription in the cell nucleus, and support the view that both speckle- and nonspeckle-associated regions of the nucleus contain sites for the coordination of transcription and splicing processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.