ArticleThe Journal of cell biology1999
Association of chromosome territories with the nuclear matrix. Disruption of human chromosome territories correlates with the release of a subset of nuclear matrix proteins.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed.
- Nuclear architecture and the structural basis of mitotic memory.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Review
- Article
- Article
- Genome-Directed Cell Nucleus Assembly.Biology · 2022Article
- Nascent RNA scaffolds contribute to chromosome territory architecture and counter chromatin compaction.Molecular cell · 2021Article
- Chromosome territories and the global regulation of the genome.Genes, chromosomes & cancer · 2019Review
- Nuclear organization mediates cancer-compromised genetic and epigenetic control.Advances in biological regulation · 2018Review
- Article
- Identification of novel nesprin-1 binding partners and cytoplasmic matrin-3 in processing bodies.Molecular biology of the cell · 2016Article
- Spatio-temporal re-organization of replication foci accompanies replication domain consolidation during human pluripotent stem cell lineage specification.Cell cycle (Georgetown, Tex.) · 2016Article
- Organization of nuclear architecture during adipocyte differentiation.Nucleus (Austin, Tex.) · 2016Review
- Nuclear matrix protein Matrin 3 is a regulator of ZAP-mediated retroviral restriction.Retrovirology · 2015Article
- Non-Random Patterns in the Distribution of NOR-Bearing Chromosome Territories in Human Fibroblasts: A Network Model of Interactions.Journal of cellular physiology · 2015Article
- Chromatin structure and replication origins: determinants of chromosome replication and nuclear organization.Journal of molecular biology · 2014Review
- Gene density and chromosome territory shape.Chromosoma · 2014Article
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- A requiem to the nuclear matrix: from a controversial concept to 3D organization of the nucleus.Chromosoma · 2014Review
- Mutations in the Matrin 3 gene cause familial amyotrophic lateral sclerosis.Nature neuroscience · 2014Article
- Alterations in replication timing of cancer-related genes in malignant human breast cancer cells.Journal of cellular biochemistry · 2013Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
To study the possible role of the nuclear matrix in chromosome territory organization, normal human fibroblast cells are treated in situ via classic isolation procedures for nuclear matrix in the absence of nuclease (e.g., DNase I) digestion, followed by chromosome painting. We report for the first time that chromosome territories are maintained intact on the nuclear matrix. In contrast, complete extraction of the internal nuclear matrix components with RNase treatment followed by 2 M NaCl results in the disruption of higher order chromosome territory architecture. Correlative with territorial disruption is the formation of a faint DNA halo surrounding the nuclear lamina and a dispersive effect on the characteristically discrete DNA replication sites in the nuclear interior. Identical results were obtained using eight different human chromosome paints. Based on these findings, we developed a fractionation strategy to release the bulk of nuclear matrix proteins under conditions where the chromosome territories are maintained intact. A second treatment results in disruption of the chromosome territories in conjunction with the release of a small subset of acidic proteins. These proteins are distinct from the major nuclear matrix proteins and may be involved in mediating chromosome territory organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.