ArticleJournal of virology1999
Cytopathogenic and noncytopathogenic RNA replicons of classical swine fever virus.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 90 citations in OpenAlex.
- Essential role ofJournal of virology · 2025Article
- New Generation Self-Replicating RNA Vaccines Derived from Pestivirus Genome.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Review
- Characteristics of Classical Swine Fever Virus Variants Derived from Live Attenuated GPEViruses · 2021Article
- Autonomously Replicating RNAs of Bungowannah Pestivirus: EJournal of virology · 2020Article
- A Polyuridine Insertion in the 3' Untranslated Region of Classical Swine Fever Virus Activates Immunity and Reduces Viral Virulence in Piglets.Journal of virology · 2020Article
- Article
- Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-Terminal Domain.Journal of virology · 2018Article
- Uncoupling of ProteaseJournal of virology · 2017Article
- Classical swine fever virus nonstructural protein p7 modulates infectious virus production.Scientific reports · 2017Article
- Npro of classical swine fever virus contributes to pathogenicity in pigs by preventing type I interferon induction at local replication sites.Veterinary research · 2014Article
- Efficient sensing of infected cells in absence of virus particles by plasmacytoid dendritic cells is blocked by the viral ribonuclease E(rns.).PLoS pathogens · 2013Article
- Interactive cellular proteins related to classical swine fever virus non-structure protein 2 by yeast two-hybrid analysis.Molecular biology reports · 2012Article
- Selection of classical swine fever virus with enhanced pathogenicity reveals synergistic virulence determinants in E2 and NS4B.Journal of virology · 2012Article
- Experimental infection of Bama miniature pigs with a highly virulent classical swine fever virus.Virology journal · 2011Article
- Classical swine fever virus NS2 protein promotes interleukin-8 expression and inhibits MG132-induced apoptosis.Virus genes · 2011Article
- Biosynthesis of classical swine fever virus nonstructural proteins.Journal of virology · 2011Article
- Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3-degrading function of Npro.Journal of virology · 2009Article
- Classical swine fever virus Npro interacts with interferon regulatory factor 3 and induces its proteasomal degradation.Journal of virology · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine the minimal requirements for autonomous RNA replication of classical swine fever virus (CSFV), genomes having in-frame deletions within the genes for structural and flanking nonstructural proteins were constructed, based on an infectious cDNA clone of CSFV Alfort/187. RNA was transcribed in vitro from the respective plasmids and transfected into SK-6 swine kidney cells. The replication competence of the RNA was determined by immunostaining transfected cells for CSFV NS3 protein and by analysis of cell extracts for viral RNA, as well as protein synthesis at different times after transfection. The genes encoding N(pro), C, E(rns), E1, E2, p7, and NS2 proved to be dispensable for RNA replication, but the efficiency of replication varied strongly between individual constructs. RNA replicons containing the complete NS2-NS3 gene persisted in transfected cells and continued to replicate without causing any obvious morphological or functional damage to the cells, whereas genomes lacking the NS2 gene replicated more efficiently and induced a cytopathic effect. These findings suggest that NS2, although it is not essential for pestivirus RNA replication, has a regulatory function therein. Both cytopathogenic and noncytopathogenic replicons were packaged into virus particles provided in trans by a cotransfected full-length helper virus genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.