ArticleJournal of bacteriology1999
In Thermoanaerobacterium thermosulfurigenes EM1 S-layer homology domains do not attach to peptidoglycan.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 60 citations in OpenAlex.
- Bacterial xylan utilization regulons: systems for coupling depolymerization of methylglucuronoxylans with assimilation and metabolism.Journal of industrial microbiology & biotechnology · 2022Article
- Comparative Phenotypic Analysis ofJournal of microbiology and biotechnology · 2021Article
- Lipoteichoic acid mediates binding of a Lactobacillus S-layer protein.Glycobiology · 2018Article
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- Identification of extracellular surface-layer associated proteins in Lactobacillus acidophilus NCFM.Microbiology (Reading, England) · 2013Article
- Are the surface layer homology domains essential for cell surface display and glycosylation of the S-layer protein from Paenibacillus alvei CCM 2051T?Journal of bacteriology · 2013Article
- Species-specific cell wall binding affinity of the S-layer proteins of mosquitocidal bacterium Bacillus sphaericus C3-41.Applied and environmental microbiology · 2009Article
- Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification.Applied and environmental microbiology · 2004Article
- Interaction of the crystalline bacterial cell surface layer protein SbsB and the secondary cell wall polymer of Geobacillus stearothermophilus PV72 assessed by real-time surface plasmon resonance biosensor technology.Journal of bacteriology · 2004Article
- Cloning, expression, and cell surface localization of Paenibacillus sp. strain W-61 xylanase 5, a multidomain xylanase.Applied and environmental microbiology · 2003Article
- The first biantennary bacterial secondary cell wall polymer and its influence on S-layer glycoprotein assembly.The Biochemical journal · 2002Article
- One repeat of the cell wall binding domain is sufficient for anchoring the Lactobacillus acidophilus surface layer protein.Journal of bacteriology · 2002Article
- Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen.Applied and environmental microbiology · 2002Article
- Cell-surface-anchoring role of N-terminal surface layer homology domains of Clostridium cellulovorans EngE.Journal of bacteriology · 2002Article
- S-Layer proteins.Journal of bacteriology · 2000Review
- Structural and functional analyses of the secondary cell wall polymer of Bacillus sphaericus CCM 2177 that serves as an S-layer-specific anchor.Journal of bacteriology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three exocellular enzymes of Thermoanaerobacterium thermosulfurigenes EM1 possess a C-terminal triplicated sequence related to a domain of bacterial cell surface proteins (S-layer proteins). At least one copy of this sequence, named the SLH (for S-layer homology) domain, is also present at the N terminus of the S-layer protein of this bacterium. The hypothesis that SLH domains serve to anchor proteins to the cell surface was investigated by using the SLH domain-containing xylanase. This enzyme was isolated from T. thermosulfurigenes EM1, and different forms with and without SLH domains were synthesized in Escherichia coli. The interaction of these proteins with isolated components of the cell envelope was determined to identify the attachment site in the cell wall. In addition, a polypeptide consisting of three SLH domains and the N terminus of the S-layer protein of T. thermosulfurigenes EM1 were included in these studies. The results indicate that SLH domains are necessary for the attachment of these proteins to peptidoglycan-containing sacculi. Extraction of the native sacculi with hydrofluoric acid led to the conclusion that not peptidoglycan but accessory cell wall polymers function as the adhesion component in the cell wall. Our results provide further evidence that attachment of proteins via their SLH domains represents an additional mode to display polypeptides on the cell surfaces of bacteria.
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