ArticleJournal of bacteriology1999
High-frequency RecA-dependent and -independent mechanisms of Congo red binding mutations in Yersinia pestis.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Plagued by the Past, Pressed by the Present: A One Health Perspective onBiomedicines · 2025Review
- Systematic analysis of spontaneous tandem genome amplification events in Yersinia pestis.PloS one · 2025Article
- Induction of Type I Interferon through a Noncanonical Toll-Like Receptor 7 Pathway during Yersinia pestis Infection.Infection and immunity · 2017Article
- Allelic Variation in Outer Membrane Protein A and Its Influence on Attachment ofFrontiers in microbiology · 2017Article
- Identification and characterization of small RNAs in Yersinia pestis.RNA biology · 2013Article
- Opposing roles for interferon regulatory factor-3 (IRF-3) and type I interferon signaling during plague.PLoS pathogens · 2012Article
- Heme binding proteins of Bartonella henselae are required when undergoing oxidative stress during cell and flea invasion.PloS one · 2012Article
- Differential control of Yersinia pestis biofilm formation in vitro and in the flea vector by two c-di-GMP diguanylate cyclases.PloS one · 2011Article
- A comprehensive study on the role of the Yersinia pestis virulence markers in an animal model of pneumonic plague.Folia microbiologica · 2011Article
- The importance of the small RNA chaperone Hfq for growth of epidemic Yersinia pestis, but not Yersinia pseudotuberculosis, with implications for plague biology.Journal of bacteriology · 2010Article
- Absence of inflammation and pneumonia during infection with nonpigmented Yersinia pestis reveals a new role for the pgm locus in pathogenesis.Infection and immunity · 2010Article
- recA mediated spontaneous deletions of the icaADBC operon of clinical Staphylococcus epidermidis isolates: a new mechanism of phenotypic variations.Antonie van Leeuwenhoek · 2008Article
- Yersinia pestis biofilm in the flea vector and its role in the transmission of plague.Current topics in microbiology and immunology · 2008Review
- Current trends in plague research: from genomics to virulence.Clinical medicine & research · 2006Review
- The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation.Journal of bacteriology · 2004Article
- The recent emergence of plague: a process of felonious evolution.Microbial ecology · 2004Review
- Temperature regulation of the hemin storage (Hms+) phenotype of Yersinia pestis is posttranscriptional.Journal of bacteriology · 2004Article
- Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis.Genome research · 2003Article
- Yersiniabactin production requires the thioesterase domain of HMWP2 and YbtD, a putative phosphopantetheinylate transferase.Infection and immunity · 2002Article
- Genome sequence of Yersinia pestis KIM.Journal of bacteriology · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar. The hmsHFRS genes required for CR binding (Crb(+)) are genetically linked to virulence-associated genes encoding a siderophore uptake system. These genes are contained in a 102-kb chromosomal pgm locus that is lost in a high-frequency deletion event, resulting in loss of the Crb(+) phenotype. We constructed a recA mutant strain of Y. pestis KIM10+ (YPRA) to test whether the high frequency Crb mutants result from a RecA-mediated deletion of the IS100-flanked pgm locus. Two Pgm-associated phenotypes (Crb(+) and pesticin sensitivity [Pst(s)]) were used as markers for the presence of the pgm locus in the RecA(+) KIM10+ and RecA(-) YPRA strains. In KIM10+, both phenotypes were lost at a very high (2 x 10(-3)) frequency, due to the deletion of the entire pgm locus. In YPRA, the Crb(+) phenotype was still lost at a high frequency (4.5 x 10(-5)), although the loss of the Pst(s) phenotype occurred at spontaneous antibiotic resistance mutation frequencies (2 x 10(-7)). These RecA-independent Crb(-) mutants were caused by mutations in both the hmsHFRS locus and in a newly identified gene, hmsT. Nonpigmented Yersinia pseudotuberculosis and Escherichia coli strains transformed with both hmsT and hmsHFRS became Crb(+). This study demonstrates that in a laboratory culture, the Crb(+) phenotype is unstable, independent of the pgm locus deletion. We propose that a lack of selection for the CR-binding ability of Y. pestis in vitro may contribute to the mutation frequencies observed at the hmsHFRS and hmsT loci.
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