ArticleJournal of bacteriology1999
The adherence-associated lipoprotein P100, encoded by an opp operon structure, functions as the oligopeptide-binding domain OppA of a putative oligopeptide transport system in Mycoplasma hominis.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Thymidine utilisation pathway is a novel phenotypic switch ofJournal of medical microbiology · 2022Article
- Convergent Evolution among Ruminant-Pathogenic Mycoplasma Involved Extensive Gene Content Changes.Genome biology and evolution · 2018Article
- Mycoplasma hominis impacts gene expression in Trichomonas vaginalis.Parasitology research · 2018Article
- Whole-Genome Characterization of Bacillus cereus Associated with Specific Disease Manifestations.Infection and immunity · 2018Article
- High quality draft genome sequence ofStandards in genomic sciences · 2018Article
- The Variable Internal Structure of the Mycoplasma penetrans Attachment Organelle Revealed by Biochemical and Microscopic Analyses: Implications for Attachment Organelle Mechanism and Evolution.Journal of bacteriology · 2017Article
- TheInfection and immunity · 2017Article
- Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection.PloS one · 2017Article
- Expression of the Oligopeptide Permease Operon of Moraxella catarrhalis Is Regulated by Temperature and Nutrient Availability.Infection and immunity · 2015Article
- Complete Genome Sequence of Mycoplasma hominis Strain Sprott (ATCC 33131), Isolated from a Patient with Nongonococcal Urethritis.Genome announcements · 2015Article
- Comparative genomic analysis of seven Mycoplasma hyosynoviae strains.MicrobiologyOpen · 2015Article
- Surface glycosaminoglycans mediate adherence between HeLa cells and Lactobacillus salivarius Lv72.BMC microbiology · 2013Article
- Host cell responses to persistent mycoplasmas--different stages in infection of HeLa cells with Mycoplasma hominis.PloS one · 2013Article
- Improved adhesive properties of recombinant bifidobacteria expressing the Bifidobacterium bifidum-specific lipoprotein BopA.Microbial cell factories · 2012Article
- In Mycoplasma hominis the OppA-mediated cytoadhesion depends on its ATPase activity.BMC microbiology · 2011Article
- The liposoluble proteome of Mycoplasma agalactiae: an insight into the minimal protein complement of a bacterial membrane.BMC microbiology · 2010Article
- Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas.PLoS genetics · 2009Article
- Implication of an outer surface lipoprotein in adhesion of Bifidobacterium bifidum to Caco-2 cells.Applied and environmental microbiology · 2008Article
- Differential expression of lipoprotein genes in Mycoplasma pneumoniae after contact with human lung epithelial cells, and under oxidative and acidic stress.BMC microbiology · 2008Article
- OppA, the ecto-ATPase of Mycoplasma hominis induces ATP release and cell death in HeLa cells.BMC microbiology · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoplasma hominis, a cell-wall-less prokaryote, was shown to be cytoadherent by the participation of a 100-kDa membrane protein (P100). To identify the gene encoding P100, peptides of P100 were partially sequenced to enable the synthesis of P100-specific oligonucleotides suitable as probes for the detection of the P100 gene. With this strategy, we identified a genomic region of about 10. 4 kb in M. hominis FBG carrying the P100 gene. Analysis of the complete deduced protein sequence suggests that P100 is expressed as a pre-lipoprotein with a structure in the N-terminal region common to peptide-binding proteins and an ATP- or GTP-binding P-loop structure in the C-terminal region. Downstream of the P100 gene, an additional four open reading frames putatively encoding the four core domains of an active transport system, OppBCDF, were localized. The organization of the P100 gene and oppBCDF in a transcriptionally active operon structure was demonstrated in Northern blot and reverse transcription-PCR analyses, as all gene-specific probes detected a common RNA of 9.5 kb. Primer extension analysis revealed that the transcriptional initiation site was localized 323 nucleotides upstream of the methionine-encoding ATG of the P100 gene. The peptide-binding character of the P100 protein was confirmed by fluorescence spectroscopy and strongly suggests that the cytoadherence-mediating lipoprotein P100 represents OppA, the substrate-binding domain of a peptide transport system in M. hominis.
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