ArticleJournal of bacteriology1999
Staphylococcus aureus cap5P encodes a UDP-N-acetylglucosamine 2-epimerase with functional redundancy.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Sensitizing methicillin-resistantAntimicrobial agents and chemotherapy · 2024Article
- A genetic regulatory see-saw of biofilm and virulence in MRSA pathogenesis.Frontiers in microbiology · 2023Review
- Crystal structure of the capsular polysaccharide-synthesis enzyme CapG from Staphylococcus aureus.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Coordination of capsule assembly and cell wall biosynthesis in Staphylococcus aureus.Nature communications · 2019Article
- CovR Regulates Streptococcus mutans Susceptibility To Complement Immunity and Survival in Blood.Infection and immunity · 2016Article
- Chemical Genetic Analysis and Functional Characterization of Staphylococcal Wall Teichoic Acid 2-Epimerases Reveals Unconventional Antibiotic Drug Targets.PLoS pathogens · 2016Article
- Characterizing non-hydrolyzing Neisseria meningitidis serogroup A UDP-N-acetylglucosamine (UDP-GlcNAc) 2-epimerase using UDP-N-acetylmannosamine (UDP-ManNAc) and derivatives.Carbohydrate research · 2016Article
- Discovery of Novel Putative Inhibitors of UDP-GlcNAc 2-Epimerase as Potent Antibacterial Agents.ACS medicinal chemistry letters · 2013Article
- A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerases.EMBO reports · 2008Article
- Characterization of a new variant of IS257 that has displaced the capsule genes within bovine isolates of Staphylococcus aureus.Infection and immunity · 2007Article
- Identification and biochemical characterization of two novel UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerases from respiratory pathogens.The Biochemical journal · 2007Article
- Identification of an essential gene of Listeria monocytogenes involved in teichoic acid biogenesis.Journal of bacteriology · 2006Article
- Immunization with Staphylococcus aureus clumping factor B, a major determinant in nasal carriage, reduces nasal colonization in a murine model.Infection and immunity · 2006Article
- Staphylococcus aureus strains that express serotype 5 or serotype 8 capsular polysaccharides differ in virulence.Infection and immunity · 2005Article
- Staphylococcus aureus capsular polysaccharides.Clinical microbiology reviews · 2004Review
- Characterization of a Bacillus subtilis thermosensitive teichoic acid-deficient mutant: gene mnaA (yvyH) encodes the UDP-N-acetylglucosamine 2-epimerase.Journal of bacteriology · 2002Article
- Construction and characterization of an acapsular mutant of Mannheimia haemolytica A1.Infection and immunity · 2002Article
- Staphylococcus aureus Cap5O has UDP-ManNAc dehydrogenase activity and is essential for capsule expression.Infection and immunity · 2001Article
- Listeriolysin O as a reporter to identify constitutive and in vivo-inducible promoters in the pathogen Listeria monocytogenes.Infection and immunity · 2000Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP5) is synthesized from a UDP-N-acetylglucosamine (UDP-GlcNAc) precursor that is epimerized to UDP-N-acetylmannosamine (UDP-ManNAc) and then oxidized to UDP-ManNAcA. We report the purification and biochemical characterization of a recombinant UDP-GlcNAc 2-epimerase encoded by S. aureus cap5P. Purified Cap5P converted approximately 10% of UDP-GlcNAc to UDP-ManNAc as detected by gas chromatography-mass spectrometry. The epimerization of UDP-GlcNAc to UDP-ManNAc occurred over a wide pH range and was unaffected by divalent cations. Surprisingly, CP5 expression in S. aureus was unaffected by insertional inactivation of cap5P. Sequence homology searches of the public S. aureus genomic databases revealed the presence of another putative UDP-GlcNAc 2-epimerase on the S. aureus chromosome that showed 61% identity to Cap5P. Redundancy of UDP-GlcNAc 2-epimerase function in S. aureus was demonstrated by cloning the cap5P homologue from strain Newman and complementing an Escherichia coli rffE mutant defective in UDP-GlcNAc 2-epimerase activity. Our results confirm the putative function of the S. aureus cap5P gene product and demonstrate the presence of a second gene on the staphylococcal chromosome with a similar function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.