ArticleMolecular biology of the cell1999
Fission yeast Pob1p, which is homologous to budding yeast Boi proteins and exhibits subcellular localization close to actin patches, is essential for cell elongation and separation.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Generation and characterization of conditional yeast mutants affecting each of the 2 essential functions of the scaffolding proteins Boi1/2 and Bem1.G3 (Bethesda, Md.) · 2022Article
- Rho Family GTPases in Fission Yeast Cytokinesis.Communicative & integrative biology · 2019Review
- The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.PloS one · 2013Article
- Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.Genetics · 2010Article
- Article
- Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.Molecular biology of the cell · 2009Article
- Genomic expression patterns in cell separation mutants of Schizosaccharomyces pombe defective in the genes sep10 ( + ) and sep15 ( + ) coding for the Mediator subunits Med31 and Med8.Molecular genetics and genomics : MGG · 2008Article
- The SH3/PH domain protein AgBoi1/2 collaborates with the Rho-type GTPase AgRho3 to prevent nonpolar growth at hyphal tips of Ashbya gossypii.Eukaryotic cell · 2006Article
- Probing the importance and potential roles of the binding of the PH-domain protein Boi1 to acidic phospholipids.BMC cell biology · 2002Article
- The Phox homology (PX) domain, a new player in phosphoinositide signalling.The Biochemical journal · 2001Review
- The Schizosaccharomyces pombe spo20(+) gene encoding a homologue of Saccharomyces cerevisiae Sec14 plays an important role in forespore membrane formation.Molecular biology of the cell · 2001Article
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3 authors at 1 institution in 1 country.
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Abstract
The fission yeast pob1 gene encodes a protein of 871 amino acids carrying an SH3 domain, a SAM domain, and a PH domain. Gene disruption and construction of a temperature-sensitive pob1 mutant indicated that pob1 is essential for cell growth. Loss of its function leads to quick cessation of cellular elongation. Pob1p is homologous to two functionally redundant Saccharomyces cerevisiae proteins, Boi1p and Boi2p, which are necessary for cell growth and relevant to bud formation. Overexpression of pob1 inhibits cell growth, causing the host cells to become round and swollen. In growing cells, Pob1p locates at cell tips during interphase and translocates near the division plane at cytokinesis. Thus, this protein exhibits intracellular dynamics similar to F-actin patches. However, Pob1p constitutes a layer, rather than patches, at growing cell tips. It generates two split discs flanking the septum at cytokinesis. The pob1-defective cells no longer elongate but swell gradually at the middle, eventually assuming a lemon-like morphology. Analysis using the pob1-ts allele revealed that Pob1p is also essential for cell separation. We speculate that Pob1p is located on growing plasma membrane, possibly through the function of actin patches, and may recruit proteins required for the synthesis of cell wall.
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