ArticleMolecular biology of the cell1999
The Cdc6 nucleotide-binding site regulates its activity in DNA replication in human cells.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 80 citations in OpenAlex.
- Reconstitution of human DNA licensing and the structural and functional analysis of key intermediates.Nature communications · 2025Article
- The expanding genetic and clinical landscape associated with Meier-Gorlin syndrome.European journal of human genetics : EJHG · 2023Review
- Replication initiation: Implications in genome integrity.DNA repair · 2021Review
- The Replication Protein Cdc6 Suppresses Centrosome Over-Duplication in a Manner Independent of Its ATPase Activity.Molecules and cells · 2017Article
- The DNA replication protein Cdc6 inhibits the microtubule-organizing activity of the centrosome.The Journal of biological chemistry · 2017Article
- DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.Nature communications · 2017Article
- MicroRNA-10a controls airway smooth muscle cell proliferation via direct targeting of the PI3 kinase pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization.Nucleic acids research · 2013Article
- Cdc6 protein obstructs apoptosome assembly and consequent cell death by forming stable complexes with activated Apaf-1 molecules.The Journal of biological chemistry · 2012Article
- Different roles of the human Orc6 protein in the replication initiation process.Cellular and molecular life sciences : CMLS · 2011Article
- Mutations in the pre-replication complex cause Meier-Gorlin syndrome.Nature genetics · 2011Article
- ATP-dependent pre-replicative complex assembly is facilitated by Adk1p in budding yeast.The Journal of biological chemistry · 2010Article
- Study of DNA replication in Drosophila using cell free in vitro system.Cell cycle (Georgetown, Tex.) · 2010Article
- HOXD13 binds DNA replication origins to promote origin licensing and is inhibited by geminin.Molecular and cellular biology · 2009Article
- ATP-dependent activation of p21WAF1/CIP1-associated Cdk2 by Cdc6.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage.Molecular biology of the cell · 2007Article
- Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells.Molecular biology of the cell · 2006Article
- Cleavage of Cdc6 by caspase-3 promotes ATM/ATR kinase-mediated apoptosis of HeLa cells.The Journal of cell biology · 2006Article
- Mutations in the NB-ARC domain of I-2 that impair ATP hydrolysis cause autoactivation.Plant physiology · 2006Article
- Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Cdc6 protein of budding yeast and its homologues in other species play an essential role in the initiation of DNA replication. A cDNA encoding a human homologue of Cdc6 (HsCdc6) has been cloned and expressed as a fusion protein in a soluble and functionally active form. The purified protein bound specifically to ATP and slowly hydrolyzed it, whereas HsCdc6 mutants containing amino acid substitutions in the Walker A or B motifs were defective. The mutant proteins retained the ability to bind HsOrc1 and HsCdc6 but displayed aberrant conformations in the presence of nucleotides. Microinjection of either mutant protein into human cells in G1 inhibited DNA replication, suggesting that ATP binding and hydrolysis by HsCdc6 are essential for DNA replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.