ArticleGene therapy1999
Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo.
Article in Gene therapy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 100 citations in OpenAlex.
- Review
- Incorporation of histone derived recombinant protein for enhanced disassembly of core-membrane structured liposomal nanoparticles for efficient siRNA delivery.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Optimization of transfection methods for Huh-7 and Vero cells: A comparative study.Cytology and genetics · 2012Article
- Peptide-mediated lipofection is governed by lipoplex physical properties and the density of surface-displayed amines.Journal of pharmaceutical sciences · 2008Article
- Aerosol-delivered programmed cell death 4 enhanced apoptosis, controlled cell cycle and suppressed AP-1 activity in the lungs of AP-1 luciferase reporter mice.Gene therapy · 2007Article
- Novel molecular approaches to cystic fibrosis gene therapy.The Biochemical journal · 2005Review
- Enhancement of transfection efficiency through rapid and noncovalent post-PEGylation of poly(dimethylaminoethyl methacrylate)/DNA complexes.Pharmaceutical research · 2004Article
- Transfection efficiency and cytotoxicity of nonviral gene transfer reagents in human smooth muscle and endothelial cells.Pharmaceutical research · 2004Article
- Coupling of importin beta binding peptide on plasmid DNA: transfection efficiency is increased by modification of lipoplex's physico-chemical properties.BMC biotechnology · 2003Article
- Construction of an EGF receptor-mediated histone H1(0)-based gene delivery system.Journal of cancer research and clinical oncology · 2003Article
- Increased receptor-mediated gene delivery to the liver by protamine-enhanced-asialofetuin-lipoplexes.Gene therapy · 2003Article
- Characterisation of LMD virus-like nanoparticles self-assembled from cationic liposomes, adenovirus core peptide mu and plasmid DNA.Gene therapy · 2002Article
- Utilization of synthetic peptides containing nuclear localization signals for nonviral gene transfer systems.Gene therapy · 2002Review
- Vectors for gene therapy of cardiovascular disease.Current cardiology reports · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cationic lipids can deliver genes efficiently in vitro, but are generally inhibited by the presence of serum, and their efficiency in vivo is much lower than in vitro. An attractive strategy is to induce strong DNA compaction by its association with proteins, before addition of lipids. However the use of whole proteins might present both production and immunological limitations. We have devised a system in which DNA is associated with short peptides derived from human histone or protamine, before the addition of a cationic lipid or polymer. Peptides strongly associating with DNA confer to such peptide-DNA-lipid particles an enhanced in vitro transfection efficiency over that observed with classical DNA/lipid lipoplexes, and particularly confer the capacity to transfect in the presence of serum. This acquisition of serum resistance is cell type-independent, and observed with all four lipopolyamines tested and polyethylenimine. Precompacting DNA with a histone H1-derived peptide enhances cationic lipid RPR 115335-mediated gene transfer in an in vivo model of Lewis lung carcinoma. Apart from their use in peptide-DNA-lipid association, such peptides could be useful as part of chimeric gene delivery vectors presenting a DNA-binding moiety that can be easily associated with other functional domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.