ArticleThe Journal of physiology1999
Basolateral K+ channel involvement in forskolin-activated chloride secretion in human colon.
Article in The Journal of physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 68 citations in OpenAlex.
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- Alternatives to animal models in gastroenterology and hepatology research.Frontiers in pharmacology · 2026Review
- Barrier-Strengthening Effects of Cannabidiol on Porcine Peyer's Patches.International journal of molecular sciences · 2025Article
- Intestinal current measurement detects age-dependent differences in CFTR function in rectal epithelium.Frontiers in pharmacology · 2025Article
- Contact-dependent, polarized acidification response during neutrophil-epithelial interactions.Journal of leukocyte biology · 2022Article
- Advances in CaExperimental and therapeutic medicine · 2020Review
- Parallel intermediate conductance KAmerican journal of physiology. Gastrointestinal and liver physiology · 2020Article
- Colonic Potassium Absorption and Secretion in Health and Disease.Comprehensive Physiology · 2018Review
- Genomic and rapid effects of aldosterone: what we know and do not know thus far.Heart failure reviews · 2017Review
- Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- Sexual dimorphism and oestrogen regulation of KCNE3 expression modulates the functional properties of KCNQ1 K⁺ channels.The Journal of physiology · 2011Article
- Electrogenic transport, oxygen consumption, and sensitivity to acute hypoxia of human colonic epithelium.International journal of colorectal disease · 2011Article
- Cyclic AMP-mediated chloride secretion is induced by prostaglandin F2alpha in human isolated colon.British journal of pharmacology · 2009Article
- Genomic priming of the antisecretory response to estrogen in rat distal colon throughout the estrous cycle.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Novel female sex-dependent actions of oestrogen in the intestine.The Journal of physiology · 2009Review
- Palmatine, a protoberberine alkaloid, inhibits both Ca(2+)- and cAMP-activated Cl(-) secretion in isolated rat distal colon.British journal of pharmacology · 2008Article
- Colonic epithelial ion transport is not affected in patients with diverticulosis.BMC gastroenterology · 2007Article
- Cyclic AMP-dependent Cl- secretion induced by thromboxane A2 in isolated human colon.The Journal of physiology · 2005Article
- Regulation of Cl- secretion by alpha2-adrenergic receptors in mouse colonic epithelium.The Journal of physiology · 2003Article
- Electrogenic ion transport in mammalian colon involves an ammonia-sensitive apical membrane K+ conductance.Digestive diseases and sciences · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
1. In this study we investigated the role of basolateral potassium transport in maintaining cAMP-activated chloride secretion in human colonic epithelium. 2. Ion transport was quantified in isolated human colonic epithelium using the short-circuit current technique. Basolateral potassium transport was studied using nystatin permeabilization. Intracellular calcium measurements were obtained from isolated human colonic crypts using fura-2 spectrofluorescence imaging. 3. In intact isolated colonic strips, forskolin and prostaglandin E2 (PGE2) activated an inward transmembrane current (ISC) consistent with anion secretion (for forskolin DeltaISC = 63.8+/-6.2 microA cm(-2), n = 6; for PGE2 DeltaISC = 34.3+/-5.2 microA cm(-2), n = 6). This current was inhibited in chloride-free Krebs solution or by inhibiting basolateral chloride uptake with bumetanide and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid DIDS). 4. The forskolin- and PGE2-induced chloride secretion was inhibited by basolateral exposure to barium (5 mM), tetrapentylammonium (10 microM) and tetraethylammonium (10 mM). 5. The transepithelial current produced under an apical to serosal K+ gradient in nystatin-perforated colon is generated at the basolateral membrane by K+ transport. Forskolin failed to activate this current under conditions of high or low calcium and failed to increase the levels of intracellular calcium in isolated crypts 6. In conclusion, we propose that potassium recycling through basolateral K+ channels is essential for cAMP-activated chloride secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.