ArticleThe Biochemical journal1999
cDNA cloning and characterization of guinea-pig leukotriene B4 receptor.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Using guinea pigs in studies relevant to asthma and COPD.Pulmonary pharmacology & therapeutics · 2008Review
- Cell-specific transcriptional regulation of human leukotriene B(4) receptor gene.The Journal of experimental medicine · 2000Article
- A second leukotriene B(4) receptor, BLT2. A new therapeutic target in inflammation and immunological disorders.The Journal of experimental medicine · 2000Article
- An in vivo approach showing the chemotactic activity of leukotriene B(4) in acute renal ischemic-reperfusion injury.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA for leukotriene B(4) (LTB(4)) receptor (BLT) was cloned from a guinea-pig leucocyte cDNA library. The cloned receptor cDNA encodes 348 amino acid residues and shares 73% identity with the amino acid sequence of human BLT. Northern blot analysis showed the highest expression of the receptor mRNA in leucocytes, followed by lung and spleen. The membrane fractions of HEK-293 and Cos-7 cells transfected with the cDNA showed specific LTB(4)-binding activities, with K(d) values of 0.27 and 0.17 nM respectively. Xenopus laevis oocytes injected with the cRNA of guinea-pig BLT showed LTB(4)-induced Cl(-) currents, indicating that the cloned receptor is functional. LTB(4) is metabolized to 20-hydroxy-LTB(4) and then to 20-carboxy-LTB(4), a transformation considered as a major inactivation pathway of the compound. Using the cloned receptor, we analysed the agonistic effects of LTB(4) and these two metabolites. 20-Carboxy-LTB(4) is a much weaker agonist, with a K(d) value higher than that of LTB(4) by three orders of magnitude, corresponding to a much weaker chemotactic activity. Although 20-hydroxy-LTB(4) is as potent as LTB(4) in inhibiting [(3)H]LTB(4) binding and cAMP formation, it is less potent than LTB(4) in the mobilization of intracellular Ca(2+) and the chemotaxis of Chinese hamster ovary cells expressing the guinea-pig BLT. The present study demonstrated that although LTB(4) and 20-hydroxy-LTB(4) bind to the receptor with similar affinities, they do differ in activating intracellular signalling.
Indexed as
Identifiers
10432303PMC1220439W2026805365What OpenQuestion holds
Registered trials
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