ArticleProceedings of the National Academy of Sciences of the United States of America1999
A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 29 citations in OpenAlex.
- Novel bacterial artificial chromosome vector pUvBBAC for use in studies of the functional genomics of Listeria spp.Applied and environmental microbiology · 2008Article
- Diversity of group II introns in the genome of Sinorhizobium meliloti strain 1021: splicing and mobility of RmInt1.Molecular genetics and genomics : MGG · 2003Article
- Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- A glutamine-amidotransferase-like protein modulates FixT anti-kinase activity in Sinorhizobium meliloti.BMC microbiology · 2001Article
- oriT-directed cloning of defined large regions from bacterial genomes: identification of the Sinorhizobium meliloti pExo megaplasmid replicator region.Journal of bacteriology · 2000Article
- Megaplasmid pRme2011a of Sinorhizobium meliloti is not required for viability.Journal of bacteriology · 2000Article
- Requirement for the enzymes acetoacetyl coenzyme A synthetase and poly-3-hydroxybutyrate (PHB) synthase for growth of Sinorhizobium meliloti on PHB cycle intermediates.Journal of bacteriology · 2000Article
- High-resolution physical map of the Sinorhizobium meliloti 1021 pSyma megaplasmid.Journal of bacteriology · 2000Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As part of the European Sinorhizobium meliloti (strain 1021) chromosome sequencing project, four genomic bacterial artificial chromosome (BAC) libraries have been constructed, one of which was mainly used for chromosome mapping. This library consists of 1,824 clones with an average insert size of 80 kilobases and represents approximately 20-fold total genome coverage [6.8 megabases (Mbs)]. PCR screening of 384 BAC clones with 447 chromosomal markers (PCR primer pairs), consisting of 73 markers representing 118 genes (40 individual genes and 78 genes clustered in 23 operons), two markers from the rrn operon (three loci), four markers from insertion sequences (approximately 16 loci) and 368 sequence-tagged sites allowed the identification of 252 chromosomal BAC clones and the construction of a high-density physical map of the whole 3.7-Mb chromosome of S. meliloti. An average of 5.5 overlapping and colinear BAC clones per marker, correlated with a low rate of deleted or rearranged clones (0.8%) indicate a solid BAC contigation and a correct mapping. Systematic BLASTX analysis of sequence-tagged site marker sequences allowed prediction of a biological function for a number of putative ORFs. Results are available at. This map, whose resolution averages one marker every 9 kilobases, should provide a valuable tool for further sequencing, functional analysis, and positional cloning.
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