ArticleProceedings of the National Academy of Sciences of the United States of America1999
A silencer element identified in Drosophila is required for imprinting of H19 reporter transgenes in mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Functional Conservation of a Developmental Switch in Mammals since the Jurassic Age.Molecular biology and evolution · 2019Article
- When Long Noncoding RNAs Meet Genome Editing in Pluripotent Stem Cells.Stem cells international · 2017Review
- Deletion of an X-inactivation boundary disrupts adjacent gene silencing.PLoS genetics · 2013Article
- Epigenetic mechanisms of genomic imprinting: common themes in the regulation of imprinted regions in mammals, plants, and insects.Genetics research international · 2012Article
- Novel cis-regulatory function in ICR-mediated imprinted repression of H19.Developmental biology · 2011Article
- A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells.Molecular and cellular biology · 2009Article
- The H19 differentially methylated region marks the parental origin of a heterologous locus without gametic DNA methylation.Molecular and cellular biology · 2004Article
- Analysis of imprinted murine Peg3 locus in transgenic mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2004Article
- Article
- Analysis of sequence upstream of the endogenous H19 gene reveals elements both essential and dispensable for imprinting.Molecular and cellular biology · 2002Article
- Methylation-dependent silencing at the H19 imprinting control region by MeCP2.Nucleic acids research · 2002Article
- Regulatory mechanisms at the mouse Igf2/H19 locus.Molecular and cellular biology · 2001Article
- Investigation of elements sufficient to imprint the mouse Air promoter.Molecular and cellular biology · 2001Article
- An upstream repressor element plays a role in Igf2 imprinting.The EMBO journal · 2001Article
- A transcriptional insulator at the imprinted H19/Igf2 locus.Genes & development · 2000Article
- H19 and Igf2 monoallelic expression is regulated in two distinct ways by a shared cis acting regulatory region upstream of H19.Genes & development · 2000Article
- Comparative genomic sequencing identifies novel tissue-specific enhancers and sequence elements for methylation-sensitive factors implicated in Igf2/H19 imprinting.Genome research · 2000Article
- Imprinting: silently crossing the boundary.Genome biology · 2000Review
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7 authors.
Funding
Abstract
The H19 gene is subject to genomic imprinting because it is methylated and repressed after paternal inheritance and is unmethylated and expressed after maternal inheritance. We recently identified a 1.1-kb control element in the upstream region of the H19 gene that functions as a cis-acting silencer element in Drosophila. Here we investigate the function of this element in mice. We demonstrate that both H19-lacZ and H19-PLAP reporter transgenes can undergo imprinting with repression and hypermethylation after paternal transmission at many integration sites. However, transgenes that were deleted for the 1.1-kb silencer element showed loss of paternal repression, but they did not show marked changes in the paternal methylation of the remaining upstream region. This study demonstrates that the 1.1-kb control element identified in Drosophila is required to silence paternally transmitted H19 minitransgenes in mice.
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