ArticleProceedings of the National Academy of Sciences of the United States of America1999
Mitotic recombination produces the majority of recessive fibroblast variants in heterozygous mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 130 citations in OpenAlex.
- An overlooked subset of Cx3cr1Journal of neuroinflammation · 2022Article
- Mitotic Antipairing of Homologous Chromosomes.Results and problems in cell differentiation · 2022Article
- Speciation of Genes and Genomes: Conservation of DNA Polymorphism by Barriers to Recombination Raised by Mismatch Repair System.Frontiers in genetics · 2022Review
- Excision of mutagenic replication-blocking lesions suppresses cancer but promotes cytotoxicity and lethality in nitrosamine-exposed mice.Cell reports · 2021Article
- Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.Nature neuroscience · 2020Article
- Somatic Mutagenesis in Mammals and Its Implications for Human Disease and Aging.Annual review of genetics · 2018Review
- Article
- Recombinant cells in the lung increase with age via de novo recombination events and clonal expansion.Environmental and molecular mutagenesis · 2017Article
- Simulated space radiation-induced mutants in the mouse kidney display widespread genomic change.PloS one · 2017Article
- SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair.The EMBO journal · 2016Article
- CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq.Genome biology · 2016Article
- Mitotic Intragenic Recombination: A Mechanism of Survival for Several Congenital Disorders of Glycosylation.American journal of human genetics · 2016Article
- Chronic centrosome amplification without tumorigenesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Chromosome microduplication in somatic cells decreases the genetic stability of human reprogrammed somatic cells and results in pluripotent stem cells.Scientific reports · 2015Article
- Inflammation-induced cell proliferation potentiates DNA damage-induced mutations in vivo.PLoS genetics · 2015Article
- Oxidative stress preferentially induces a subtype of micronuclei and mediates the genomic instability caused by p53 dysfunction.Mutation research · 2014Article
- Rosa26-GFP direct repeat (RaDR-GFP) mice reveal tissue- and age-dependence of homologous recombination in mammals in vivo.PLoS genetics · 2014Article
- Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress.Mutation research · 2012Article
- Ionizing radiation is a potent inducer of mitotic recombination in mouse embryonic stem cells.Mutation research · 2011Article
- Multinucleated giant cells from fibroblast cultures.Biomaterials · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Mice heterozygous at Aprt (adenine phosphoribosyltransferase) were used as a model to study in vivo loss of heterozygosity (LOH) in normal fibroblasts. Somatic cell variants that exhibited functional loss of the wild-type Aprt in vivo were recovered as APRT-deficient cell colonies after culturing in selection medium containing 2, 6-diaminopurine (DAP), an adenine analog that is toxic only to cells with APRT enzyme activity. DAP-resistant (DAP(r)) fibroblast variants were recovered at a median frequency of 12 x 10(-5) from individual ears from progeny of crosses between mouse strains 129/Sv and C3H/HeJ. The frequency of DAP(r) variants varied greatly among individual ears, suggesting that they preexisted in vivo and arose at various times during development. Polymorphic molecular markers and a cytological marker on the centromere of chromosome 8 made it possible to discriminate between each of six possible mechanistic pathways of LOH. The majority (about 80%) of the DAP(r) variants were a consequence of mitotic recombination. The prevalence of mitotic recombination in regions proximal to Aprt did not correlate with meiotic map distances. In particular, there was a higher than expected frequency of crossovers within the interval 59 cM to 67 cM. The high spontaneous frequency of Aprt LOH, mediated primarily by mitotic recombination, is fully consistent with our previous results with human peripheral T cells from individuals known to be heterozygous at APRT. Thus, this Aprt heterozygote mouse is a valid model for studying somatic mutagenesis and mitotic recombination in vivo.
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