ArticleProceedings of the National Academy of Sciences of the United States of America1999
Allele-specific activators and inhibitors for kinesin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 55 citations in OpenAlex.
- Designing Allele-Specific Inhibitors of Spastin, a Microtubule-Severing AAA Protein.Journal of the American Chemical Society · 2019Article
- Loss-of-function mutations in KIF14 cause severe microcephaly and kidney development defects in humans and zebrafish.Human molecular genetics · 2019Article
- The Bump-and-Hole Tactic: Expanding the Scope of Chemical Genetics.Cell chemical biology · 2018Review
- Chemical genetic inhibition of DEAD-box proteins using covalent complementarity.Nucleic acids research · 2018Article
- Allocating dissipation across a molecular machine cycle to maximize flux.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Engineered kinesin motor proteins amenable to small-molecule inhibition.Nature communications · 2016Article
- Analog sensitive chemical inhibition of the DEAD-box protein DDX3.Protein science : a publication of the Protein Society · 2016Article
- Microtubules are required for efficient epithelial tight junction homeostasis and restoration.American journal of physiology. Cell physiology · 2014Article
- Bioorthogonal profiling of protein methylation (BPPM) using an azido analog of S-adenosyl-L-methionine.Current protocols in chemical biology · 2013Article
- Bioorthogonal profiling of protein methylation using azido derivative of S-adenosyl-L-methionine.Journal of the American Chemical Society · 2012Article
- Article
- Expanding cofactor repertoire of protein lysine methyltransferase for substrate labeling.ACS chemical biology · 2011Article
- Frontal affinity chromatography-mass spectrometry useful for characterization of new ligands for GPR17 receptor.Journal of medicinal chemistry · 2010Article
- A small molecule inhibitor selective for a variant ATP-binding site of the chaperonin GroEL.Bioorganic & medicinal chemistry letters · 2009Article
- Formation of microtubule-based traps controls the sorting and concentration of vesicles to restricted sites of regenerating neurons after axotomy.The Journal of cell biology · 2007Article
- Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe.Nature protocols · 2007Article
- Microtubules regulate disassembly of epithelial apical junctions.BMC cell biology · 2006Article
- Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis.The Journal of biological chemistry · 2003Article
- Orphan kinesin NOD lacks motile properties but does possess a microtubule-stimulated ATPase activity.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Members of the kinesin superfamily are force-generating ATPases that drive movement and influence cytoskeleton organization in cells. Often, more than one kinesin is implicated in a cellular process, and many kinesins are proposed to have overlapping functions. By using conventional kinesin as a model system, we have developed an approach to activate or inhibit a specific kinesin allele in the presence of other similar motor proteins. Modified ATP analogs are described that do not activate either conventional kinesin or another superfamily member, Eg5. However, a kinesin allele with Arg-14 in its nucleotide binding pocket mutated to alanine can use a subset of these nucleotide analogs to drive microtubule gliding. Cyclopentyl-ATP is one such analog. Cyclopentyl-adenylylimidodiphosphate, a nonhydrolyzable form of this analog, inhibits the mutant allele in microtubule-gliding assays, but not wild-type kinesin or Eg5. We anticipate that the incorporation of kinesin mutants and allele-specific activators and inhibitors in in vitro assays should clarify the role of individual motor proteins in complex cellular processes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.