ArticleProceedings of the National Academy of Sciences of the United States of America1999
Induction of an acrosomal process in Toxoplasma gondii: visualization of actin filaments in a protozoan parasite.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Who cites it
37 citing papers in PubMed, 97 citations in OpenAlex.
- Highly divergent apicomplexan cytoskeletons provide additional models for actin biology.The FEBS journal · 2025Review
- Myosin F controls actin organization and dynamics inMolecular biology of the cell · 2024Article
- Emergent actin flows explain distinct modes of gliding motility.Nature physics · 2024Article
- Review
- Origin and arrangement of actin filaments for gliding motility in apicomplexan parasites revealed by cryo-electron tomography.Nature communications · 2023Article
- Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes ofeLife · 2019Article
- Three F-actin assembly centers regulate organelle inheritance, cell-cell communication and motility ineLife · 2019Article
- Lateral diffusion and signaling of receptor for advanced glycation end-products (RAGE): a receptor involved in chronic inflammation.European biophysics journal : EBJ · 2018Article
- Direct measurement of cortical force generation and polarization in a living parasite.Molecular biology of the cell · 2017Article
- Comparative Plasmodium gene overexpression reveals distinct perturbation of sporozoite transmission by profilin.Molecular biology of the cell · 2016Article
- Plasmodium actin is incompletely folded by heterologous protein-folding machinery and likely requires the native Plasmodium chaperonin complex to enter a mature functional state.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Actin-Dynamics in Plant Cells: The Function of Actin-Perturbing Substances: Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Disassembly activity of actin-depolymerizing factor (ADF) is associated with distinct cellular processes in apicomplexan parasites.Molecular biology of the cell · 2015Article
- Synthetic chondramide A analogues stabilize filamentous actin and block invasion by Toxoplasma gondii.Journal of natural products · 2013Article
- The enigma of eugregarine epicytic folds: where gliding motility originates?Frontiers in zoology · 2013Article
- Toxofilin upregulates the host cortical actin cytoskeleton dynamics, facilitating Toxoplasma invasion.Journal of cell science · 2012Article
- Critical role for heat shock protein 20 (HSP20) in migration of malarial sporozoites.The Journal of biological chemistry · 2012Article
- Spatial localisation of actin filaments across developmental stages of the malaria parasite.PloS one · 2012Article
- Cytoskeleton assembly in Toxoplasma gondii cell division.International review of cell and molecular biology · 2012Review
- Solution structure and dynamics of ADF from Toxoplasma gondii.Journal of structural biology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The invasive stages of Toxoplasma gondii, an Apicomplexan parasite, actively invade their host cells in an actin-dependent way. However, despite containing biochemically significant amounts of actin, actin filaments have never been observed in these parasites. Jasplakinolide, a membrane-permeable actin-polymerizing and filament-stabilizing drug, induced the polymerization of actin filaments at the anterior end of each tachyzoite in association with the conoid, where they formed, in many cases, a prominent membrane-enclosed apical projection reminiscent of acrosomal processes of invertebrate sperm. These jasplakinolide-induced filaments decorated with myosin subfragment 1, demonstrating unequivocally that they were indeed actin. Jasplakinolide-treated tachyzoites were unable to invade host cells, but once the drug was removed the parasites were able to enter host cells. Actin polymerization at the apical end of the parasite is consistent with the role of the apical end in host-cell invasion powered by a jackhammer-like extension and retraction of the conoid complex coupled to the secretion and rearward capping of surface proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.