ArticleThe Journal of clinical investigation1999
Role of the tyrosine kinase pyk2 in the integrin-dependent activation of human neutrophils by TNF.
Article in The Journal of clinical investigation, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 128 citations in OpenAlex.
- The mechano-immunological landscape in the tumor microenvironment: From mechanical sensing to a new therapeutic paradigm.Materials today. Bio · 2026Review
- Kinase regulation by liquid-liquid phase separation.Trends in cell biology · 2023Review
- Tyrphostin A9 attenuates glioblastoma growth by suppressing PYK2/EGFR-ERK signaling pathway.Journal of neuro-oncology · 2023Article
- STAT3 regulates inflammatory cytokine production downstream of TNFR1 by inducing expression of TNFAIP3/A20.Journal of cellular and molecular medicine · 2022Article
- Disease severity-specific neutrophil signatures in blood transcriptomes stratify COVID-19 patients.Genome medicine · 2021Article
- Sphingomyelin synthase 2, but not sphingomyelin synthase 1, is involved in HIV-1 envelope-mediated membrane fusion.The Journal of biological chemistry · 2014Article
- Role of focal adhesion tyrosine kinases in GPVI-dependent platelet activation and reactive oxygen species formation.PloS one · 2014Article
- Monosodium urate activates Src/Pyk2/PI3 kinase and cathepsin dependent unconventional protein secretion from human primary macrophages.Molecular & cellular proteomics : MCP · 2013Article
- PYK2: a calcium-sensitive protein tyrosine kinase activated in response to fertilization of the zebrafish oocyte.Developmental biology · 2013Article
- LPS-induced release of IL-6 from glia modulates production of IL-1β in a JAK2-dependent manner.Journal of neuroinflammation · 2012Article
- Functional properties of granulocytes after thermal injury.Immunologic research · 2012Review
- The β-glucan receptor Dectin-1 activates the integrin Mac-1 in neutrophils via Vav protein signaling to promote Candida albicans clearance.Cell host & microbe · 2011Article
- Live cell imaging of paxillin in rolling neutrophils by dual-color quantitative dynamic footprinting.Microcirculation (New York, N.Y. : 1994) · 2011Article
- Pyk2 is required for neutrophil degranulation and host defense responses to bacterial infection.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Recruitment of Pyk2 to SHPS-1 signaling complex is required for IGF-I-dependent mitogenic signaling in vascular smooth muscle cells.Cellular and molecular life sciences : CMLS · 2010Article
- Hematopoietic Pyk2 regulates migration of differentiated HL-60 cells.Journal of inflammation (London, England) · 2010Article
- Pyk2 uncouples metabotropic glutamate receptor G protein signaling but facilitates ERK1/2 activation.Molecular brain · 2010Article
- Calpain inhibition impairs TNF-alpha-mediated neutrophil adhesion, arrest and oxidative burst.Molecular immunology · 2010Article
- Regulation of TNF-induced oxygen radical production in human neutrophils: role of delta-PKC.Journal of leukocyte biology · 2010Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Secretion of inflammatory products from neutrophils can be induced by a combination of signals from ligated integrins and receptors for soluble, physiological agonists such as TNF. Here we identify pyk2 in primary human neutrophils; localize it to focal adhesions and podosomes; and demonstrate its tyrosine phosphorylation, activation, and association with paxillin during stimulation of adherent cells by TNF. Tyrphostin A9 emerged as the most potent and selective of 51 tyrosine kinase inhibitors tested against the TNF-induced respiratory burst. Tyrphostin A9 inhibited TNF-induced tyrosine phosphorylation of pyk2 without blocking the cells' bactericidal activity. Wortmannin, an inhibitor of phosphatidylinositol-3-kinase, potently blocked the TNF-induced respiratory burst and selectively inhibited tyrosine phosphorylation of pyk2. Thus, pyk2 appears to play an essential role in the ability of neutrophils to integrate signals from beta(2) integrins and TNF receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.